antibody

Anti-Nitric Oxide Synthase, Brain(1-181) NOS1 Antibody (Monoclonal, NOS-B1)

MA1072

100μg/vial

monoclonal

mouse

nonconjugated

NOS-B1

The same clone is also sold as:

• Abnova: MAB1470

human, rat

Anti-Nitric Oxide Synthase, Brain(1-181) NOS1 Antibody (Monoclonal, NOS-B1)

100μg/vial

Monoclonal

NOS-B1

Mouse

Goat, Human, Pig, Rat

WB

Western blot, 0.5µg/ml, Human, goat, pig, rat.

Other applications have not been tested. Optimal dilutions should be determined by end users.

Boster Bio Anti-Nitric Oxide Synthase, Brain (1-181) NOS1 Antibody (Monoclonal, NOS-B1) catalog # MA1072. Tested in WB applications. This antibody reacts with Goat, Human, Pig, Rat.

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

NOS1

P29476

Recombinant neuronal NOS fragment (amino acids 1-181) from rat brain.

Lyophilized

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Ascites

No cross reactivity with other proteins

Mouse IgG1

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Nitric oxide synthase, brain

Nitric oxide synthase, brain; 1.14.13.39; BNOS; Constitutive NOS; NC-NOS; N

Nitric oxide synthase, brain

160559 MW

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Inhibitory transmitter for non-adrenergic and non-cholinergic nerves in the colorectum. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR. Inhibitory transmitter for non- adrenergic and non-cholinergic nerves in the colorectum.

Cell membrane, sarcolemma ; Peripheral membrane protein. Cell projection, dendritic spine. In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex (By similarity). In neurons, enriched in dendritic spines.

Isoform N-NOS-1 is expressed in brain and colorectum. Found in the Auerbach's plexus of the enteric nervous system. Isoform PNNOS is expressed in the penis, urethra, prostate, and skeletal muscle, and coexists with the cerebellar nnos in the pelvic plexus, bladder and liver, and is detectable in the cerebellum.

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot.

Belongs to the NOS family.

Nitric Oxide Synthase 1 (NOS1,neuronal NOS,nNOS1) is a messenger molecule, mediating the effect of endothelium-derived relaxing factor in blood vessels and the cytotoxic actions of macrophages, and playing a part in neuronal communication in the brain. It may be involved in neuronal cell death and damage in neurological illness. nNOS1 localized to the 12q24.2 region of human chromosome 12. It splice variant, expressed in testis, that encodes an NH2-terminal truncated protein of 1098 amino acids. nNOS cDNA clones were shown to contain different 5' terminal exons spliced to a common exon 2. Genomic cloning and sequence analysis demonstrate that the unique exons are positioned within 300 bp of each other but separated from exon 2 by an intron that is at least 20 kb in length. The neuronal isoform of nitric oxide synthase is highly expressed in mammalian skeletal muscle, it suggested a specific role for NOS1 in the local metabolic inhibition of alpha-adrenergic vasoconstriction in active skeletal muscle. The novel gaseous neuromediator nitric oxide is though to play an important role in development and plasticity. Despite this, gene-knockout mice lacking neuronal (Type I) nitric oxide synthase exhibit relatively normal brain development and behavior.