Anti-NEFH Antibody (Monoclonal, N52) | Boster MA1071 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-NEFH Antibody (Monoclonal, N52)

catalog :

MA1071

quantity :

100μg/vial

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

N52

The same clone is also sold as:

MilliporeSigma: N0142

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

product information

SKU :

MA1071

Product Name :

Anti-NEFH Antibody (Monoclonal, N52)

Size :

100μg/vial

Clonality :

Monoclonal

Clone Number :

N52

Host :

Mouse

Reactivity :

Human, Mouse, Rat

Application(s) :

IHC, WB

Application Details :

Immunohistochemistry (Paraffin-embedded Section), 1-2µg/ml, Human, mouse, rat, By Heat. Immunohistochemistry (Frozen Section), 1-2µg/ml, Human, mouse, rat Western blot, 0.5ml, Human, mouse, rat.

Application Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-NEFH Antibody (Monoclonal, N52) catalog # MA1071. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Concentration :

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Gene Name :

NEFH

Uniprot ID :

P16884

Immunogen :

C-terminal segment of enzymatically dephosphorylated pig Neurofilament 200.

Form :

Lyophilized

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Cross-reactivity :

No cross reactivity with other proteins

Isotype :

Mouse IgG1

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Gene Full Name :

Neurofilament heavy polypeptide

Synonyms :

Neurofilament heavy polypeptide; NF-H; 200 kDa neurofilament protein; Neurofilament triplet H protein; Nefh; Nfh;

Protein Name :

Neurofilament heavy polypeptide

Molecular Weight :

115378 MW

Protein Function :

Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).

Sequence Similarities :

Belongs to the intermediate filament family.

Background :

Neurofilaments are composed of 3 neuron-specific proteins with apparent molecular masses of 68 kD (NFL), 125 kD (NFM), and 200 kD (NFH) on SDS-gel electrophoresis. Genomic clones for the largest human neurofilament protein (NF-H) were isolated, the intron/exon boundaries mapped and the entire protein-coding regions (exons) sequenced. mutations in neurofilaments have been linked to some forms of Charcot-Marie-Tooth disease (CMT).

Research Category :

Alzheimer'S Disease, Cell Adhesion Proteins, Cell Type Marker, Cytoskeleton, Cytoskeleton / Ecm, Intermediate Filaments, Neurodegenerative Disease, Neurology Process, Neuron Marker, Neuroscience, Signal Transduction