Anti-NF68 Nefl Antibody (Monoclonal, NR4) | Boster MA1070 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-NF68 Nefl Antibody (Monoclonal, NR4)

catalog :

MA1070

quantity :

100μg/vial

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

NR4

The same clone is also sold as:

Novus Biologicals: NBP2-47969-0.1mg, NBP2-44891-0.1mg
Santa Cruz Biotechnology: sc-58560
MilliporeSigma: N5139

reactivity :

human, rat

application :

western blot, immunohistochemistry

product information

SKU :

MA1070

Product Name :

Anti-NF68 Nefl Antibody (Monoclonal, NR4)

Size :

100μg/vial

Clonality :

Monoclonal

Clone Number :

NR4

Host :

Mouse

Reactivity :

Human, Pig, Rat

Application(s) :

IHC, WB

Application Details :

Immunohistochemistry (Paraffin-embedded Section), 2-4µg/ml, Human, pig, rat, By Heat. Immunohistochemistry (Frozen Section), 2-4µg/ml, Human, pig, rat Western blot, 1-2µg/ml, Human, pig, rat.

Application Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-NF68 Nefl Antibody (Monoclonal, NR4) catalog # MA1070. Tested in IHC, WB applications. This antibody reacts with Human, Pig, Rat.

Concentration :

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Gene Name :

NEFL

Uniprot ID :

P19527

Immunogen :

Pig spinal cord.

Form :

Lyophilized

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Cross-reactivity :

No cross reactivity with other proteins

Isotype :

Mouse IgG1

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Gene Full Name :

Neurofilament light polypeptide

Synonyms :

Neurofilament light polypeptide; NF-L; 68 kDa neurofilament protein; Neurofilament triplet L protein; Nefl; Nf68, Nfl;

Protein Name :

Neurofilament light polypeptide

Molecular Weight :

61335 MW

Protein Function :

Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).

Sequence Similarities :

Belongs to the intermediate filament family.

Background :

Neurofilaments are composed of 3 neuron-specific proteins with apparent molecular masses of 68 kD (NFL), 125 kD (NFM) and 200 kD (NFH) on SDS-gel electrophoresis. And they have a role in the maturation of regenerating myelinated axons. Neurofilament 68 (NF68), also called Neurofilament Protein, Light Chain (NFL). It is one of the most abundant cytoskeletal components of the neuron. Mutations in this gene were reported as a cause for autosomal dominant Charcot-Marie-Tooth type 2E (CMT2E) linked to chromosome 8p21. NFL was identified repeatedly in both screenings and found to interact with Myotubularin-related 2 gene, MTMR2 in both Schwann cells and neurons.

Research Category :

Cell Type Marker, Cytoskeleton, Cytoskeleton / Ecm, Developmental Biology, Intermediate Filaments, Lineage Markers, Lineage Specification, Neurodegenerative Disease, Neurology Process, Neuron Marker, Neuroscience, Signal Transduction, Stem Cells