Anti-N-Cadherin-2 CDH2 CD325-Monoclonal Antibody | Boster MA1067 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-N-Cadherin-2 CDH2 CD325-Monoclonal Antibody

catalog :

MA1067

quantity :

100μg/vial

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

GC-4

The same clone is also sold as:

Novus Biologicals: NBP2-21805-0.05ml

reactivity :

human, mouse, rat, chicken

application :

western blot, immunohistochemistry

product information

SKU :

MA1067

Product Name :

Anti-N-Cadherin-2 CDH2 CD325-Monoclonal Antibody

Size :

100μg/vial

Clonality :

Monoclonal

Clone Number :

GC-4

Host :

Mouse

Reactivity :

Chicken, Human, Mouse, Rabbit, Rat

Predicted Reactivity :

Monkey

Application(s) :

IHC, WB

Application Details :

Immunohistochemistry (Frozen Section), 4µg/ml, Human, mouse, rat, rabbit, chicken Western blot, 2µg/ml, Human, mouse, rat, rabbit, chicken.

Application Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-N-Cadherin-2 CDH2 CD325-Monoclonal Antibody catalog # MA1067. Tested in IHC, WB applications. This antibody reacts with Chicken, Human, Mouse, Rabbit, Rat.

Concentration :

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Gene Name :

CDH2

Uniprot ID :

Q9Z1Y3

Immunogen :

Affinity purified chicken heart A-CAM.

Form :

Lyophilized

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Cross-reactivity :

No cross reactivity with other proteins

Isotype :

Mouse IgG1

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Gene Full Name :

Cadherin-2

Synonyms :

Cadherin-2; Neural cadherin; N-cadherin; CD325; Cdh2;

Protein Name :

Cadherin-2

Molecular Weight :

99686 MW

Protein Function :

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density (By similarity).

Subcellular Localization :

Cell membrane ; Single-pass type I membrane protein.

Tissue Specificity :

In testis, expressed in Sertoli and germ cells.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F).

Background :

N-cadherin (NCAD) is a member of the cadherin cell-cell adhesion receptor family that includes P-, E-, and R-cadherin and liver cell adhesion molecule (L-CAM). N-Cadherin,, also known as Cadherin-2, encodes a 907-amino acid protein that includes a 159-amino acid signal sequence. Human and mouse nucleotide sequences are 96% identical. Mouse Ncad gene consists of 16 exons dispersed over more than 200 kb of genomic DNA. Human N-cadherin gene contains 16 exons and its sequence is highly similar to both the mouse NCAD gene (including the large first and second introns) and other cadherin genes. N-cadherin is mapped to 18q11.2. Cadherin regulates dendritic spine morphogenesis.

Research Category :

Cadherins, Cell Adhesion, Cytoskeleton / Ecm, Signal Transduction