Anti-Myosin(Skeletal, Slow) Myh7 Antibody (Monoclonal, NOQ7.5.4D)

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-Myosin(Skeletal, Slow) Myh7 Antibody (Monoclonal, NOQ7.5.4D)

catalog :

MA1064

quantity :

100μg/vial

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

NOQ7.5.4D

The same clone is also sold as:

MilliporeSigma: M8421
Abcam: ab11083
Invitrogen: MA5-32986

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

product information

SKU :

MA1064

Product Name :

Anti-Myosin(Skeletal, Slow) Myh7 Antibody (Monoclonal, NOQ7.5.4D)

Size :

100μg/vial

Clonality :

Monoclonal

Clone Number :

NOQ7.5.4D

Host :

Mouse

Reactivity :

Human, Mouse, Rabbit, Rat

Application(s) :

IHC, WB

Application Details :

Immunohistochemistry (Paraffin-embedded Section), 1-2µg/ml, Human, mouse, rabbit, rat, By Heat. Western blot, 0.5-2µg/ml, Human, mouse, rabbit, rat.

Application Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-Myosin (Skeletal, Slow) Myh7 Antibody (Monoclonal, NOQ7.5.4D) catalog # MA1064. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rabbit, Rat.

Concentration :

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Gene Name :

MYH7

Uniprot ID :

P12883

Immunogen :

Human skeletal muscle myosin purified from myofibrils.

Form :

Lyophilized

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Cross-reactivity :

No cross reactivity with other proteins

Isotype :

Mouse IgG1

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Gene Full Name :

Myosin-7

Synonyms :

Myosin-7; Myosin heavy chain 7; Myosin heavy chain slow isoform; MyHC-slow; Myosin heavy chain, cardiac muscle beta isoform; MyHC-beta; Myh7;

Protein Name :

Myosin-7

Molecular Weight :

223097 MW

Protein Function :

Myosins are actin-based motor molecules with ATPase activity essential for muscle contraction. Forms regular bipolar thick filaments that, together with actin thin filaments, constitute the fundamental contractile unit of skeletal and cardiac muscle.

Subcellular Localization :

Cytoplasm, myofibril. Thick filaments of the myofibrils.

Tissue Specificity :

MYH7: Both wild type and variant Gln-403 aredetected in skeletal muscle (at protein level).

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).

Sequence Similarities :

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.

Background :

Myosin is composed of 2 heavy chains of about 200,000 daltons each and 4 light chains of about 20,000 daltons each. Skeletal Myosin (slow),, also known as light chain 3 (MYL3), mapped to 3p. Fodor et al. (1989) found that the MYL3 gene has 7 exons, the last of which is completely untranslated 3-prime sequence.

Research Category :

Cytoskeleton, Cytoskeleton / Ecm, Motor Proteins, Myosin, Signal Transduction