Anti-P-GlycoProtein(MDR) ABCB4 Antibody (Monoclonal, F4)

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-P-GlycoProtein(MDR) ABCB4 Antibody (Monoclonal, F4)

catalog :

MA1060

quantity :

100μg/vial

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

F4

The same clone is also sold as:

Invitrogen: MA5-13854
MilliporeSigma: P7965

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry

product information

SKU :

MA1060

Product Name :

Anti-P-GlycoProtein(MDR) ABCB4 Antibody (Monoclonal, F4)

Size :

100μg/vial

Clonality :

Monoclonal

Clone Number :

Host :

Mouse

Reactivity :

Human

Application(s) :

IHC, ICC, WB

Application Details :

Immunohistochemistry (Paraffin-embedded Section), 1-2µg/ml, Human, By Heat. Immunocytochemistry, 1µg/ml, HumanImmunohistochemistry (Frozen Section), 1-2µg/ml, Human Western blot, 0.5-1µg/ml, Human.

Application Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-P-GlycoProtein (MDR) ABCB4 Antibody (Monoclonal, F4) catalog # MA1060. Tested in IHC, ICC, WB applications. This antibody reacts with Human.

Concentration :

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Gene Name :

Abcb1a

Uniprot ID :

P21447

Immunogen :

Mixture of human and hamster drug-resistant whole cells and crude plasma membranes.

Form :

Lyophilized

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Cross-reactivity :

No cross reactivity with other proteins

Isotype :

Mouse IgG1

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Gene Full Name :

Multidrug resistance protein 1A

Synonyms :

Multidrug resistance protein 1A; 3.6.3.44; ATP-binding cassette sub-family B member 1A; MDR1A; Multidrug resistance protein 3; P-glycoprotein 3; Abcb1a; Abcb4, Mdr1a, Mdr3, Pgy-3, Pgy3;

Protein Name :

Multidrug resistance protein 1

Molecular Weight :

140647 MW

Protein Function :

Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.

Subcellular Localization :

Cell membrane; Multi-pass membrane protein.

Tissue Specificity :

ABCB1: Expressed in liver, kidney, small intestineand brain.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P), IHC(F) and ICC.

Sequence Similarities :

Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.

Background :

P-Glycoprotein,, also known as Multidrug Resistance 1 (MDR1), is one of the ATP-binding cassette transporters family. P-glycoprotein-1 is involved in the transport of 3 of these protease inhibitors in vitro. MDR1 gene is mapped to the 7q21.1 by in situ hybridization. The MDR1 gene product, P-glycoprotein, mediates the transport of the cardiac glycoside, digoxin.

Research Category :

Atpases, Cancer, Lipid Metabolism, Metabolism, Nuclear Import / Export, Plasma Membrane, Protein Trafficking, Signal Transduction