Anti-Mcm7 Antibody (Monoclonal, DCS-141) | Boster MA1058 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-Mcm7 Antibody (Monoclonal, DCS-141)

catalog :

MA1058

quantity :

100μg/vial

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

DCS-141

The same clone is also sold as:

Santa Cruz Biotechnology: sc-65469

reactivity :

human, rat

application :

western blot, immunocytochemistry

product information

SKU :

MA1058

Product Name :

Anti-Mcm7 Antibody (Monoclonal, DCS-141)

Size :

100μg/vial

Clonality :

Monoclonal

Clone Number :

DCS-141

Host :

Mouse

Reactivity :

Human

Application(s) :

ICC, WB

Application Details :

Immunocytochemistry , 1µg/ml, Human Western blot, 0.5-1µg/ml, Human.

Application Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-Mcm7 Antibody (Monoclonal, DCS-141) catalog # MA1058. Tested in ICC, WB applications. This antibody reacts with Human.

Concentration :

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Gene Name :

MCM7

Uniprot ID :

Q6AYN8

Immunogen :

Recombinant human Mcm7.

Form :

Lyophilized

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Cross-reactivity :

No cross reactivity with other proteins

Isotype :

Mouse IgG1

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Gene Full Name :

DNA helicase

Synonyms :

DNA helicase ; 3.6.4.12 ; Mcm7 ; rCG_55961 ;

Protein Name :

DNA replication licensing factor MCM7

Molecular Weight :

81063 MW

Protein Function :

MCM7: Acts as component of the MCM2-7 complex (MCM complex)which is the putative replicative helicase essential for 'once percell cycle' DNA replication initiation and elongation ineukaryotic cells. The active ATPase sites in the MCM2-7 ring areformed through the interaction surfaces of two neighboringsubunits such that a critical structure of a conserved argininefinger motif is provided in trans relative to the ATP-binding siteof the Walker A box of the adjacent subunit. The six ATPase activesites, however, are likely to contribute differentially to thecomplex helicase activity. Required for S-phase checkpointactivation upon UV-induced damage.

Subcellular Localization :

Nucleus.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for ICC.

Sequence Similarities :

Belongs to the MCM family.

Background :

Initiation of DNA replication is a complex process involving the concerted action of many proteins. The chromosome 7-linked gene referred to by Nakatsuru et al. as MCM2 has been designated MCM7. The MCM2-7 complex is comprised of 6 subunits, MCM2 through MCM7, and is a ring-shaped heterohexameric ATPase involved in DNA replication. MCM7 is located in 7q21.3-q22.1.

Research Category :

DNA / RNA, DNA Synthesis, Epigenetics and Nuclear Signaling