Anti-HSP27 Hspb1 Monoclonal Antibody | Boster MA1048 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-HSP27 Hspb1 Monoclonal Antibody

catalog :

MA1048

quantity :

100μg/vial

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

IAP-9

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

product information

SKU :

MA1048

Product Name :

Anti-HSP27 Hspb1 Monoclonal Antibody

Size :

100μg/vial

Clonality :

Monoclonal

Clone Number :

IAP-9

Host :

Mouse

Reactivity :

Human, Mouse, Rabbit, Rat

Application(s) :

IHC, ICC, WB

Application Details :

Immunohistochemistry (Paraffin-embedded Section), 1-2µg/ml, Human, mouse, rabbit, rat, By Heat. Immunocytochemistry, 1µg/ml, Human, mouse, rabbit, ratImmunohistochemistry (Frozen Section), 1-2µg/ml, Human, mouse, rabbit, rat Western blot, 0.5-2µg/ml, Human, mouse, rabbit, rat.

Application Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-HSP27 Hspb1 Monoclonal Antibody catalog # MA1048. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rabbit, Rat.

Concentration :

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Gene Name :

HSPB1

Uniprot ID :

P42930

Immunogen :

Partially purified inhibitor of actin polymerization (IAP) protein from turkey gizzard smooth muscle.

Form :

Lyophilized

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Cross-reactivity :

No cross reactivity with other proteins

Isotype :

Mouse IgG1

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Gene Full Name :

Heat shock protein beta-1

Synonyms :

Heat shock protein beta-1; HspB1; Heat shock 27 kDa protein; HSP 27; Hspb1; Hsp27;

Protein Name :

Heat shock protein beta-1

Molecular Weight :

22893 MW

Protein Function :

Involved in stress resistance and actin organization.

Subcellular Localization :

Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, spindle. Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles (By similarity).

Tissue Specificity :

Expressed in a variety of tissues. High levels in lung, adrenal, xiphoid, adipose tissue, heart and striated and smooth muscle, lower levels in the CNS. Adult levels are much higher in the slow-twitch soleus muscle than in the fast-twitch rectus femoris and extensor digitorum muscles.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P), IHC(F) and ICC.

Sequence Similarities :

Belongs to the small heat shock protein (HSP20) family.

Background :

The heat-shock proteins (HSPs) belong to a larger group of polypeptides, the stress proteins, that are induced in various combinations in response to environmental challenges and developmental transitions. Heat-shock 27-kD protein1also knows as HSPB. Synthesis of the small (27-kD) HSP has been shown to be correlated with the acquisition of thermotolerance. HSP27 gene is mapped to 7q11.23. Mutant small heat-shock protein 27 causes axonal Charcot-Marie-Tooth disease and distal hereditary motor neuropathy. Heat shock protein 27 prevents cellular polyglutamine toxicity and suppresses the increase of reactive oxygen species caused by huntingtin.

Research Category :

Atherosclerosis, Cancer, Cardiovascular, Chaperones, Contractility, Heat Shock Proteins, Protein Trafficking, Signal Transduction, Tumor Biomarkers