This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Boster
product type :
antibody
product name :
Anti-GFAP Antibody (Monoclonal, G-A-5)
catalog :
MA1045
quantity :
100μg/vial
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
G-A-5

The same clone is also sold as:
reactivity :
human, rat
application :
western blot, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
product information
SKU :
MA1045
Product Name :
Anti-GFAP Antibody (Monoclonal, G-A-5)
Size :
100μg/vial
Clonality :
Monoclonal
Clone Number :
G-A-5
Host :
Mouse
Reactivity :
Human, Pig, Rat
Application(s) :
IF, IHC-P, IHC-F, WB
Application Details :
Western blot, 0.5-1µg/ml, Human, pig, rat. Immunohistochemistry (Paraffin-embedded Section), 0.4-1µg/ml, Human, pig, rat, By Heat. Immunohistochemistry (Frozen Section), 0.5-1µg/ml, Human, pig, rat. Immunofluorescence, 2µg/ml, Rat.
Application Notes :
Other applications have not been tested. Optimal dilutions should be determined by end users.
Description :
Boster Bio Anti-GFAP Antibody (Monoclonal, G-A-5) catalog # MA1045. Tested in IF, IHC-P, IHC-F, WB applications. This antibody reacts with Human, Pig, Rat.
Concentration :
Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.
Gene Name :
GFAP
Uniprot ID :
P47819
Immunogen :
GFAP from pig spinal cord.
Form :
Lyophilized
Contents :
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Purification :
Ascites
Cross-reactivity :
No cross reactivity with other proteins
Isotype :
Mouse IgG1
Storage :
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Reconstitution :
Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Gene Full Name :
Glial fibrillary acidic protein
Synonyms :
Glial fibrillary acidic protein; GFAP; Gfap;
Protein Name :
Glial fibrillary acidic protein
Molecular Weight :
49957 MW
Protein Function :
GFAP, a class-III intermediate filament, is a cell- specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Subcellular Localization :
Cytoplasm. Associated with intermediate filaments.
Tissue Specificity :
Expressed in the cortex and hippocampus. Expression decreases following acute and chronic corticosterone treatment.
Recommended Detection Systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).
Sequence Similarities :
Belongs to the intermediate filament family.
Background :
Glial fibrillary acidic protein (GFAP) is an intermediate filament protein of 52Kda. GFAP gene is mapped to human 17q21. GFAP is a useful marker of astroglia in the brain. Mutations in GFAP, encoding glial fibrillary acidic protein, are associated with Alexander disease.
Research Category :
Cell Adhesion Proteins, Cell Type Marker, Cell Type Markers, Cytoskeleton, Cytoskeleton / Ecm, Intermediate Filaments, Neural Stem Cells, Neuroscience, Signal Transduction, Stem Cells, Tags & Cell Markers
company information
Boster
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
https://www.bosterbio.com
925.485.4527
headquarters: USA
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