Anti-Cyclin D2 Ccnd2 Antibody (Monoclonal, DCS-3) | Boster MA1034 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-Cyclin D2 Ccnd2 Antibody (Monoclonal, DCS-3)

catalog :

MA1034

quantity :

100μg/vial

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

DCS-3

The same clone is also sold as:

Santa Cruz Biotechnology: sc-56305

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry

product information

SKU :

MA1034

Product Name :

Anti-Cyclin D2 Ccnd2 Antibody (Monoclonal, DCS-3)

Size :

100μg/vial

Clonality :

Monoclonal

Clone Number :

DCS-3

Host :

Mouse

Reactivity :

Human, Mouse

Application(s) :

ICC, WB

Application Details :

Immunocytochemistry , 1µg/ml, Human, mouse Western blot, 2-4µg/ml, Human, mouse.

Application Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-Cyclin D2 Ccnd2 Antibody (Monoclonal, DCS-3) catalog # MA1034. Tested in ICC, WB applications. This antibody reacts with Human, Mouse.

Concentration :

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Gene Name :

CCND2

Uniprot ID :

Q04827

Immunogen :

Recombinant human cyclin D2 protein.

Form :

Lyophilized

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Cross-reactivity :

No cross reactivity with other proteins

Isotype :

Mouse IgG2a

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Gene Full Name :

G1/S-specific cyclin-D2

Synonyms :

G1/S-specific cyclin-D2; Vin-1 proto-oncogene; Ccnd2; Vin-1;

Protein Name :

G1/S-specific cyclin-D2

Molecular Weight :

32826 MW

Protein Function :

Regulatory component of the cyclin D2-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G (1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G (1) phase. Hypophosphorylates RB1 in early G (1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D2/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex (By similarity).

Subcellular Localization :

Nucleus. Cytoplasm. Membrane. Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated into the nucleus through interaction with KIP/CIP family members.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for ICC.

Sequence Similarities :

Belongs to the cyclin family. Cyclin D subfamily.

Background :

Inaba et al. (1992) used murine cDNA clones for 3 cyclin D genes that are normally expressed during the G1 phase of the cell cycle to clone the cognate human genes. D-type cyclins (cyclins D1, D2, and D3) are regarded as essential links between cell environment and the core cell cycle machinery. cyclin D2 (CCND2) gene ia assigned to 12p13.

Research Category :

Cancer, Cell Biology, Cell Cycle, Epigenetics and Nuclear Signaling