Anti-Cyclin D1 Ccnd1 Antibody (Monoclonal, DCS-6) | Boster MA1033 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-Cyclin D1 Ccnd1 Antibody (Monoclonal, DCS-6)

catalog :

MA1033

quantity :

100μg/vial

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

DCS-6

The same clone is also sold as:

OriGene: AM32864PU-T, AM32864PU-S
Exbio: 1P-166-C025, 11-166-C025, 11-166-C100, 1P-166-C100
BioLegend: 681902
Abnova: MAB11280, MAB14208, MAB1449, MAB1851
Santa Cruz Biotechnology: sc-20044
MilliporeSigma: C7464
Novus Biologicals: NBP2-33138, NBP2-15189-0.1mg
Invitrogen: MA5-43999, MA5-44125, MA1-24750, MA5-44141, MA5-28534, AHF0082
US Biological: 138535
BD Biosciences: 556470

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

product information

SKU :

MA1033

Product Name :

Anti-Cyclin D1 Ccnd1 Antibody (Monoclonal, DCS-6)

Size :

100μg/vial

Clonality :

Monoclonal

Clone Number :

DCS-6

Host :

Mouse

Reactivity :

Human, Mouse, Rat

Application(s) :

IHC, ICC, WB

Application Details :

Immunohistochemistry (Paraffin-embedded Section), 8µg/ml, Human, mouse, rat, By Heat. Immunocytochemistry, 1µg/ml, Human, mouse, ratImmunohistochemistry (Frozen Section), 8µg/ml, Human, mouse, rat Western blot, 4µg/ml, Human, mouse, rat.

Application Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-Cyclin D1 Ccnd1 Antibody (Monoclonal, DCS-6) catalog # MA1033. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Concentration :

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Gene Name :

CCND1

Uniprot ID :

P39948

Immunogen :

Recombinant human cyclin D1 protein.

Form :

Lyophilized

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Cross-reactivity :

No cross reactivity with other proteins

Isotype :

Mouse IgG2a

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Gene Full Name :

G1/S-specific cyclin-D1

Synonyms :

G1/S-specific cyclin-D1; Ccnd1;

Protein Name :

G1/S-specific cyclin-D1

Molecular Weight :

33483 MW

Protein Function :

Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G (1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G (1) phase. Hypophosphorylates RB1 in early G (1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex, Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner (By similarity).

Subcellular Localization :

Nucleus. Cytoplasm. Membrane. Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated into the nucleus through interaction with KIP/CIP family members.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P), IHC(F) and ICC.

Sequence Similarities :

Belongs to the cyclin family. Cyclin D subfamily.

Background :

D-type cyclins (cyclins D1, D2, and D3) are regarded as essential links between cell environment and the core cell cycle machinery. CyclinD1 (CCND1), also known as BCL1. Expression of cyclin D1, but not of cyclins A and E, was induced by transfection of the RB gene into RB-deficient tumor cells. CCND1 gene to 11q13. cyclin D1 gene can function as an oncogene. Cyclin D1 expression is regulated by the retinoblastoma protein.