Anti-Collagen, Type IV COL4A1 Antibody (Monoclonal, COL-94)

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-Collagen, Type IV COL4A1 Antibody (Monoclonal, COL-94)

catalog :

MA1030

quantity :

100μg/vial

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

COL-94

The same clone is also sold as:

Santa Cruz Biotechnology: sc-59814
OriGene: DM229, DM229-05
Invitrogen: MA1-22148
MilliporeSigma: C1926

reactivity :

human

application :

immunohistochemistry

product information

SKU :

MA1030

Product Name :

Anti-Collagen, Type IV COL4A1 Antibody (Monoclonal, COL-94)

Size :

100μg/vial

Clonality :

Monoclonal

Clone Number :

COL-94

Host :

Mouse

Reactivity :

Human

Application(s) :

IHC

Application Details :

Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Human, By Heat. Immunohistochemistry (Frozen Section), 0.5-1µg/ml, Human.

Application Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-Collagen, Type IV COL4A1 Antibody (Monoclonal, COL-94) catalog # MA1030. Tested in IHC applications. This antibody reacts with Human.

Concentration :

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Gene Name :

COL4A1; COL4A3; COL4A4; COL4A5; COL4A6

Uniprot ID :

P02462

Immunogen :

Human collagen type IV.

Form :

Lyophilized

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Cross-reactivity :

No cross reactivity with other proteins

Isotype :

Mouse IgG1

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Gene Full Name :

Collagen alpha-1(IV) chain

Synonyms :

Collagen alpha-1 (IV) chain; Arresten; COL4A1;

Protein Name :

Collagen alpha-1(IV) chain; Collagen alpha-3(IV) chain; Collagen alpha-4(IV) chain; Collagen alpha-5(IV) chain; Collagen alpha-6(IV) chain

Molecular Weight :

160615 MW

Protein Function :

Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.

Subcellular Localization :

Secreted, extracellular space, extracellular matrix, basement membrane.

Tissue Specificity :

Highly expressed in placenta.

Recommended Detection Systems :

Boster recommends HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).

Sequence Similarities :

Belongs to the type IV collagen family.

Background :

Collagen IV is comprised of a third chain (alpha-3) together with the 2 classical ones, alpha-1 and alpha-2. COL4A3 gene is localized to 2q36-q37 by analysis of somatic cell hybrids and by in situ hybridization. The alpha-3 chain of type IV collagen induces autoimmune Goodpasture syndrome. Mutations in the COL4A4 and COL4A3 genes cause familial benign hematuria.

Research Category :

Cancer, Collagen, Cytoskeleton / Ecm, Ecm Proteins, Extracellular Matrix, Invasion/Microenvironment, Mesenchymal Stem Cells, Signal Transduction, Stem Cells