Anti-c-Myc Antibody (Monoclonal, 9E10) | Boster MA1028 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-c-Myc Antibody (Monoclonal, 9E10)

catalog :

MA1028

quantity :

100μg/vial

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

90000000000

The same clone is also sold as:

MilliporeSigma: M5546
Abcam: ab197139, ab72468

reactivity :

human, rat

application :

western blot, immunohistochemistry, immunocytochemistry

product information

SKU :

MA1028

Product Name :

Anti-c-Myc Antibody (Monoclonal, 9E10)

Size :

100μg/vial

Clonality :

Monoclonal

Clone Number :

90000000000

Host :

Mouse

Reactivity :

Human

Predicted Reactivity :

Monkey, Rabbit

Application(s) :

IHC, ICC, WB

Application Details :

Immunohistochemistry (Paraffin-embedded Section), 8µg/ml, Human, By Heat. Immunocytochemistry, 1µg/ml, Human Western blot, 4µg/ml, Human.

Application Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-c-Myc Antibody (Monoclonal, 9E10) catalog # MA1028. Tested in IHC, ICC, WB applications. This antibody reacts with Human.

Concentration :

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Gene Name :

MYC

Uniprot ID :

P09416

Immunogen :

Synthetic peptide corresponding to residues 408-439 of the human p62c-Myc protein.

Form :

Lyophilized

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Cross-reactivity :

No cross reactivity with other proteins

Isotype :

Mouse IgG1

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Gene Full Name :

Myc proto-oncogene protein

Synonyms :

Myc proto-oncogene protein; Proto-oncogene c-Myc; Transcription factor p64; Myc;

Protein Name :

Myc proto-oncogene protein

Molecular Weight :

48898 MW

Protein Function :

Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'- CAC[GA]TG-3'. Activates the transcription of growth-related genes.

Subcellular Localization :

Nucleus, nucleoplasm. Nucleus, nucleolus.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.

Sequence Similarities :

Contains 1 bHLH (basic helix-loop-helix) domain.

Background :

C-Myc is an oncogene that functions both in the stimulation of cell proliferation and in apoptosis. c-Myc elicits its oncogenic activity by causing immortalization, and to a lesser extent the transformation of cells, in addition to several other mechanisms. The c-MYC proto-oncogene encodes a transcription factor that is critical for cell growth and proliferation. It is one of the genes frequently altered in cancer cells in which it exhibits constitutive activity. Downregulation of c-Myc is critical for 2-Methoxyestradiol (2ME2)-induced oxidative stress and apoptosis in AML cells. And its up-regulation is important for promoting lymphocyte cell division, and demonstrating that GFP-c-Myc expression is a marker of proliferating lymphocytes in vivo.

Research Category :

Cancer, Cell Cycle, Cell Differentiation, Domain Families, Epigenetics and Nuclear Signaling, Hlh / Leucine Zipper, Nuclear, Oncoproteins, Oncoproteins/Suppressors, Signaling Pathways, Stem Cells, Tgf Beta, Transcription, Transcription Factors, Tumor Biomarkers