Anti-Cytokeratin Peptide 18 KRT18 Antibody (Monoclonal, CY-90)

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-Cytokeratin Peptide 18 KRT18 Antibody (Monoclonal, CY-90)

catalog :

MA1026

quantity :

100μg/vial

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

CY-90

The same clone is also sold as:

MilliporeSigma: C8541

reactivity :

human, rat

application :

western blot, immunohistochemistry

product information

SKU :

MA1026

Product Name :

Anti-Cytokeratin Peptide 18 KRT18 Antibody (Monoclonal, CY-90)

Size :

100μg/vial

Clonality :

Monoclonal

Clone Number :

CY-90

Host :

Mouse

Reactivity :

Human, Rat

Application(s) :

IHC, WB

Application Details :

Immunohistochemistry (Paraffin-embedded Section), 1-2µg/ml, Human, rat, By Heat. Immunohistochemistry (Frozen Section), 0.5-2µg/ml, Human, rat Western blot, 0.5-1µg/ml, Human, rat.

Application Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-Cytokeratin Peptide 18 KRT18 Antibody (Monoclonal, CY-90) catalog # MA1026. Tested in IHC, WB applications. This antibody reacts with Human, Rat.

Concentration :

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Gene Name :

KRT18

Uniprot ID :

Q5BJY9

Immunogen :

The human epidermal carcinoma A-431 and MCF-7 human breast cancer cell lines.

Form :

Lyophilized

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Cross-reactivity :

No cross reactivity with other proteins

Isotype :

Mouse IgG1

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Gene Full Name :

Keratin, type I cytoskeletal 18

Synonyms :

Keratin, type I cytoskeletal 18; Cytokeratin-18; CK-18; Keratin-18; K18; Krt18 ; Krt1-18 ;

Protein Name :

Keratin, type I cytoskeletal 18

Molecular Weight :

47761 MW

Protein Function :

When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection (By similarity). Involved in the uptake of thrombin-antithrombin complexes by hepatic cells.

Subcellular Localization :

Nucleus matrix. Nucleus, nucleolus. Cytoplasm.

Tissue Specificity :

Expressed on the plasma membrane of hepatocytes and in the narrow apical portions of supporting cells in the vomeronasal sensory epithelium. Detected in the type III alveolar cells of the lung, in the proliferative crypt epithelium of the small intestine and in the older intragemmal cells of the tongue.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).

Sequence Similarities :

Belongs to the intermediate filament family.

Background :

Intermediate filaments (IFs) are a structurally related family of cellular proteins that appear to be intimately involved with the cytoskeleton. Human keratin 18 (KRT18) and the homologous mouse Endo B are type I IF protein subunits whose expression is restricted in adults to a variety of simple epithelial tissues. The KRT18 gene is 3,791 bp long and the keratin 18 protein is coded for by 7 exons. The K18 gene is 3791 bp in length and the K18 protein is coded for by seven exons. By Southern blotting using the genomic DNA PCR product, the gene for keratin 18 is assigned to chromosome 12. Mutation of human keratin 18 in association with cryptogenic cirrhosis.

Research Category :

Cell Type Markers, Class I, Cytoskeleton, Cytoskeleton / Ecm, Ecm Proteins, Extracellular Matrix, Intermediate Filaments, Microtubules, Signal Transduction, Structures, Tumor Associated