Anti-Cytokeratin Peptide 7 KRT7 Antibody (Monoclonal, LDS-68)

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-Cytokeratin Peptide 7 KRT7 Antibody (Monoclonal, LDS-68)

catalog :

MA1024

quantity :

100μg/vial

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

LDS-68

The same clone is also sold as:

Abnova: MAB1442

reactivity :

human, rat

application :

western blot, immunohistochemistry

product information

SKU :

MA1024

Product Name :

Anti-Cytokeratin Peptide 7 KRT7 Antibody (Monoclonal, LDS-68)

Size :

100μg/vial

Clonality :

Monoclonal

Clone Number :

LDS-68

Host :

Mouse

Reactivity :

Human

Application(s) :

IHC, WB

Application Details :

Immunohistochemistry (Paraffin-embedded Section), 2-4µg/ml, Human, By Heat. Immunohistochemistry (Frozen Section), 2-4µg/ml, Human Western blot, 1-2µg/ml, Human.

Application Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-Cytokeratin Peptide 7 KRT7 Antibody (Monoclonal, LDS-68) catalog # MA1024. Tested in IHC, WB applications. This antibody reacts with Human.

Concentration :

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Gene Name :

KRT7

Uniprot ID :

Q6IG12

Immunogen :

Cytoskeletal preparation of the RT4 human bladder carcinoma cell line.

Form :

Lyophilized

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Cross-reactivity :

No cross reactivity with other proteins

Isotype :

Mouse IgG1

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Gene Full Name :

Keratin, type II cytoskeletal 7

Synonyms :

Keratin, type II cytoskeletal 7; Cytokeratin-7; CK-7; Keratin-7; K7; Type-II keratin Kb7; Krt7 ; Kb7 , Krt2-7 ;

Protein Name :

Keratin, type II cytoskeletal 7

Molecular Weight :

50709 MW

Protein Function :

Blocks interferon-dependent interphase and stimulates DNA synthesis in cells.

Subcellular Localization :

KRT7: Cytoplasm

Tissue Specificity :

KRT7: Expressed in cultured epidermal, bronchial andmesothelial cells but absent in colon, ectocervix and liver.Observed throughout the glandular cells in the junction betweenstomach and esophagus but is absent in the esophagus.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).

Sequence Similarities :

Belongs to the intermediate filament family.

Background :

KRT7 is a type II keratin of simple nonkeratinizing epithelia. The deduced 489-amino acid protein has a calculated molecular mass of about 54 kD. K7 contains 4 central alpha-helical segments with heptad repeats of hydrophobic residues characteristic of a coiled-coil region. Within this domain, K7 shares 73% homology with epidermal K6B. KRT7 gene contains 9 exons and spans more than 15.6 kb. K7 gene is mapped to chromosome 12. Keratin 7 is expressed in a wide range of epithelial structures in humans.

Research Category :

Class I, Cytoskeleton, Cytoskeleton / Ecm, Intermediate Filaments, Signal Transduction