Anti-Cdk7/CAK Antibody (Monoclonal, MO-1.1) | Boster MA1022 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-Cdk7/CAK Antibody (Monoclonal, MO-1.1)

catalog :

MA1022

quantity :

100μg/vial

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

Mo-1.1

The same clone is also sold as:

Abnova: MAB1440
OriGene: AM09103PU-N

reactivity :

human, rat

application :

western blot, immunohistochemistry, immunocytochemistry

product information

SKU :

MA1022

Product Name :

Anti-Cdk7/CAK Antibody (Monoclonal, MO-1.1)

Size :

100μg/vial

Clonality :

Monoclonal

Clone Number :

MO-1.1

Host :

Mouse

Reactivity :

Human

Predicted Reactivity :

Monkey

Application(s) :

IHC, ICC, WB

Application Details :

Immunocytochemistry , 1µg/ml, HumanImmunohistochemistry (Frozen Section), 0.5µg/ml, Human Western blot, 0.25µg/ml, Human.

Application Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-Cdk7/CAK Antibody (Monoclonal, MO-1.1) catalog # MA1022. Tested in IHC, ICC, WB applications. This antibody reacts with Human.

Concentration :

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Gene Name :

CDK7

Uniprot ID :

P51952

Immunogen :

Recombinant human Cdk7 protein.

Form :

Lyophilized

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Cross-reactivity :

No cross reactivity with other proteins

Isotype :

Mouse IgG2b

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Gene Full Name :

Cyclin-dependent kinase 7

Synonyms :

Cyclin-dependent kinase 7; 2.7.11.22; 2.7.11.23; 39 protein kinase; P39 Mo15; CDK-activating kinase 1; Cell division protein kinase 7; TFIIH basal transcription factor complex kinase subunit; Cdk7; Cak, Cak1, Mo15;

Protein Name :

Cyclin-dependent kinase 7

Molecular Weight :

37141 MW

Protein Function :

Serine/threonine kinase involved in cell cycle control and in RNA polymerase II-mediated RNA transcription. Cyclin- dependent kinases (CDKs) are activated by the binding to a cyclin and mediate the progression through the cell cycle. Each different complex controls a specific transition between 2 subsequent phases in the cell cycle. Required for both activation and complex formation of CDK1/cyclin-B during G2-M transition, and for activation of CDK2/cyclins during G1-S transition (but not complex formation). CDK7 is the catalytic subunit of the CDK-activating kinase (CAK) complex. Phosphorylates SPT5/SUPT5H, SF1/NR5A1, POLR2A, p53/TP53, CDK1, CDK2, CDK4, CDK6 and CDK11B/CDK11. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation, thus regulating cell cycle progression. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Phosphorylation of POLR2A in complex with DNA promotes transcription initiation by triggering dissociation from DNA. Its expression and activity are constant throughout the cell cycle. Upon DNA damage, triggers p53/TP53 activation by phosphorylation, but is inactivated in turn by p53/TP53; this feedback loop may lead to an arrest of the cell cycle and of the transcription, helping in cell recovery, or to apoptosis. Required for DNA-bound peptides-mediated transcription and cellular growth inhibition (By similarity).

Subcellular Localization :

Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Colocalizes with PRKCI in the cytoplasm and nucleus. Translocates from the nucleus to cytoplasm and perinuclear region in response to DNA-bound peptides (By similarity).

Tissue Specificity :

CDK7: Ubiquitous.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F) and ICC.

Sequence Similarities :

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

Background :

CDK-activating kinases (CAKs) are multisubunit proteins that phosphorylate and thus activate certain cyclin-dependent protein kinases in the regulation of cell cycle progression. Cyclin dependent kinase7 (CDK7) gene is mapped to chromosome 2p15-cen. CDK7 functions in both cyclin binding and T-loop phosphorylation and that these 2 steps of CDK1 activation are mutually dependent.

Research Category :

Cell Biology, Cell Cycle, Epigenetics and Nuclear Signaling, Kinases/Phosphatases