Anti-CDK4 DRD3 Antibody (Monoclonal, DCS-31) | Boster MA1020 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-CDK4 DRD3 Antibody (Monoclonal, DCS-31)

catalog :

MA1020

quantity :

100μg/vial

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

DCS-31

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry

product information

SKU :

MA1020

Product Name :

Anti-CDK4 DRD3 Antibody (Monoclonal, DCS-31)

Size :

100μg/vial

Clonality :

Monoclonal

Clone Number :

DCS-31

Host :

Mouse

Reactivity :

Human, Mouse, Rat

Application(s) :

ICC, WB

Application Details :

Immunocytochemistry , 1µg/ml, Human, mouse, rat Western blot, 0.5-1µg/ml, Human, mouse, rat.

Application Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-CDK4 DRD3 Antibody (Monoclonal, DCS-31) catalog # MA1020. Tested in ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Concentration :

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Gene Name :

CDK4

Uniprot ID :

P35462

Immunogen :

Recombinant human Cdk4 protein.

Form :

Lyophilized

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Cross-reactivity :

No cross reactivity with other proteins

Isotype :

Mouse IgG2a

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Gene Full Name :

D(3) dopamine receptor

Synonyms :

D (3) dopamine receptor; Dopamine D3 receptor; DRD3;

Protein Name :

Cyclin-dependent kinase 4

Molecular Weight :

44225 MW

Protein Function :

Dopamine receptor whose activity is mediated by G proteins which inhibit adenylyl cyclase. Promotes cell proliferation.

Subcellular Localization :

Cell membrane ; Multi-pass membrane protein. Both membrane-bound and scattered in the cytoplasm during basal conditions. Receptor stimulation results in the rapid internalization and sequestration of the receptors at the perinuclear area (5 and 15 minutes), followed by the dispersal of the receptors to the membrane (30 minutes). DRD3 and GRK4 co- localize in lipid rafts of renal proximal tubule cells.

Tissue Specificity :

Brain

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for ICC.

Sequence Similarities :

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

Background :

Cyclin-dependent kinase-4 (CDK4) is a protein-serine kinase involved in the cell cycle. Human cell division is regulated primarily at the G1-to-S or the G2-to-M boundaries within the cell cycle. The complexes formed by CDK4 and the D-type cyclins are involved in the control of cell proliferation during the G1 phase. CDK4 is inhibited by p16, also known as cyclin-dependent kinase inhibitor-2. CDK4 is mapped to 12q14. CDK4 expression and activity are required for cytokine responsiveness in T cells.

Research Category :

Neuroscience, Neurotransmission, Receptors / Channels