Anti-CDC6 Antibody (Monoclonal, DCS-180) | Boster MA1019 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-CDC6 Antibody (Monoclonal, DCS-180)

catalog :

MA1019

quantity :

100μg/vial

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

DCS-180

The same clone is also sold as:

Invitrogen: MA5-14314
Santa Cruz Biotechnology: sc-56273
Abnova: MAB1437

reactivity :

human, mouse

application :

western blot

product information

SKU :

MA1019

Product Name :

Anti-CDC6 Antibody (Monoclonal, DCS-180)

Size :

100μg/vial

Clonality :

Monoclonal

Clone Number :

DCS-180

Host :

Mouse

Reactivity :

Human

Application(s) :

Application Details :

Western blot, 1-2µg/ml, Human.

Application Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-CDC6 Antibody (Monoclonal, DCS-180) catalog # MA1019. Tested in WB applications. This antibody reacts with Human.

Concentration :

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Gene Name :

CDC6

Uniprot ID :

O89033

Immunogen :

Recombinant human Cdc6.

Form :

Lyophilized

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Cross-reactivity :

No cross reactivity with other proteins

Isotype :

Mouse IgG1

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Gene Full Name :

Cell division control protein 6 homolog

Synonyms :

Cell division control protein 6 homolog; CDC6-related protein; p62 (cdc6); Cdc6;

Protein Name :

Cell division control protein 6 homolog

Molecular Weight :

62614 MW

Protein Function :

Involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated.

Subcellular Localization :

Nucleus. Cytoplasm. The protein is nuclear in G1 and cytoplasmic in S-phase cells.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot.

Sequence Similarities :

Belongs to the CDC6/cdc18 family.

Background :

In yeasts, Cdc6 (Saccharomyces cerevisiae) and Cdc18 (Schizosaccharomyces pombe) associate with the origin recognition complex (ORC) proteins to render cells competent for DNA replication. Cdc6 is overexpressed in human cancers, where it has a critical regulatory role in addition to DNA replication. Yan et al. (1998) showed that Cdc6 is expressed selectively in proliferating but not quiescent mammalian cells, both in culture and within tissues in intact animals. During the transition from a growth-arrested to a proliferative state, transcription of mammalian Cdc6 is regulated by E2F proteins, as revealed by a functional analysis of the human Cdc6 promoter and by the ability of exogenously expressed E2F proteins to stimulate the endogenous Cdc6 gene. They conclude that expression of human Cdc6 is regulated in response to mitogenic signals though transcriptional control mechanisms involving E2F proteins, and that Cdc6 is required for initiation of DNA replication in mammalian cells.

Research Category :

Cell Biology, Cell Cycle, Cell Division, DNA / RNA, DNA Synthesis, Epigenetics and Nuclear Signaling