Anti-CD3 CD3E Antibody (Monoclonal, CA-3) | Boster MA1015 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-CD3 CD3E Antibody (Monoclonal, CA-3)

catalog :

MA1015

quantity :

100μg/vial

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

CA-3

The same clone is also sold as:

Abnova: MAB1433
OriGene: AM20601PU-N

reactivity :

human, rat

application :

immunohistochemistry, immunocytochemistry, flow cytometry

product information

SKU :

MA1015

Product Name :

Anti-CD3 CD3E Antibody (Monoclonal, CA-3)

Size :

100μg/vial

Clonality :

Monoclonal

Clone Number :

CA-3

Host :

Mouse

Reactivity :

Human

Application(s) :

Flow Cytometry, IHC, ICC

Application Details :

Immunohistochemistry (Frozen Section), 1µg/ml, Human.Immunocytochemistry, 0.5-1µg/ml, Human. Flow Cytometry, 1-3μg/1x10^6 cells, Human

Application Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-CD3 CD3E Antibody (Monoclonal, CA-3) catalog # MA1015. Tested in Flow Cytometry, IHC, ICC applications. This antibody reacts with Human.

Concentration :

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Gene Name :

CD3E

Uniprot ID :

D4A5M2

Immunogen :

Human thymocytes followed by Sezary T cells.

Form :

Lyophilized

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Cross-reactivity :

No cross reactivity with other proteins

Isotype :

Mouse IgG1

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Gene Full Name :

CD3e molecule, epsilon(CD3-TCR complex)

Synonyms :

CD3 antigen, epsilon polypeptide (Predicted) ; Protein Cd3e ; Cd3e ; Cd3e_predicted ; rCG_58471 ;

Protein Name :

T-cell surface glycoprotein CD3 epsilon chain

Molecular Weight :

21312 MW

Protein Function :

CD3E: The CD3 complex mediates signal transduction.

Subcellular Localization :

CD3E: Membrane; Single-pass type I membraneprotein. Cd3e: Cell membrane

Recommended Detection Systems :

Boster recommends HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F) and ICC.

Sequence Similarities :

Contains 1 Ig-like (immunoglobulin-like) domain.

Background :

The alpha-beta heterodimeric T-cell antigen receptor binds antigen in association with major histocompatibility complex proteins on host cell surfaces. These 2 disulfide-linked glycoproteins, TCRA and TCRB are associated on T-cell surfaces with a complex of proteins called CD3 (formerly T3). Human CD3 consists of at least 4 proteins: gamma, delta, epsilon, and zeta. The CD3-gamma/CD3-delta gene pair is within 300 kb of the CD3-epsilon gene and therefore these genes form a tightly linked cluster on 11q23. The clustering may be significant in terms of their simultaneous activations during T-cell development.

Research Category :

Adaptive Immunity, Hematopoietic Progenitors, Immunology, Stem Cells, T Cells