Anti-Cathepsin L Ctsl Antibody (Monoclonal, 33/2) | Boster MA1014 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-Cathepsin L Ctsl Antibody (Monoclonal, 33/2)

catalog :

MA1014

quantity :

100μg/vial

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

33/2

The same clone is also sold as:

Invitrogen: MA1-26774

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

product information

SKU :

MA1014

Product Name :

Anti-Cathepsin L Ctsl Antibody (Monoclonal, 33/2)

Size :

100μg/vial

Clonality :

Monoclonal

Clone Number :

33/2

Host :

Mouse

Reactivity :

Human, Mouse, Rat

Application(s) :

IHC, WB

Application Details :

Immunohistochemistry (Frozen Section), 4µg/ml, Human, mouse, rat Western blot, 2µg/ml, Human, mouse, rat.

Application Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-Cathepsin L Ctsl Antibody (Monoclonal, 33/2) catalog # MA1014. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Concentration :

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Gene Name :

CTSL1

Uniprot ID :

P07154

Immunogen :

Procathepsin L isolated from the human lung cancer cell line EPLC 32M1.

Form :

Lyophilized

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Cross-reactivity :

No cross reactivity with other proteins

Isotype :

Mouse IgG1

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Gene Full Name :

Cathepsin L1

Synonyms :

Cathepsin L1; 3.4.22.15; Cathepsin L; Cyclic protein 2; CP-2; Major excreted protein; MEP; Procathepsin L; Cathepsin L1 heavy chain; Cathepsin L1 light chain; Ctsl; Ctsl1;

Protein Name :

Cathepsin L1

Molecular Weight :

37660 MW

Protein Function :

Important for the overall degradation of proteins in lysosomes. Procathepsin L is required for maximal stimulation of steroidogenesis by TIMP1.

Subcellular Localization :

Lysosome

Tissue Specificity :

Both mature cathepsin L1 and procathepsin L are found in the upper epidermis. The lower epidermis predominantly contains procathepsin L. In seminiferous tubules expression is greater at stages VI-VII than at stages IX-XII.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F).

Sequence Similarities :

Belongs to the peptidase C1 family.

Background :

Cathepsin L is a lysosomal cysteine proteinase with a major role in intracellular protein catabolism. It also shows the most potent collagenolytic and elastinolytic activity in vitro of any of the cathepsins. Cathepsin L has been implicated in pathologic processes including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. Human liver cathepsin L consists of a heavy chain of about 25 kD and a light chain of about 5 kD. The gene is mapped to 9q21-q22. Cathepsin L is required for endothelial progenitor cell-induced neovascularization.

Research Category :

Cell Adhesion Proteins, Cytoskeleton / Ecm, Ecm Enzymes, Extracellular Matrix, Membrane Proteins, Neuroscience, Signal Transduction