Anti-Caldesmon (Smooth) Cald1 Antibody (Monoclonal, hHCD)

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-Caldesmon (Smooth) Cald1 Antibody (Monoclonal, hHCD)

catalog :

MA1009

quantity :

100μg/vial

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

hHCD

reactivity :

mouse, rat

application :

western blot, immunohistochemistry

product information

SKU :

MA1009

Product Name :

Anti-Caldesmon (Smooth) Cald1 Antibody (Monoclonal, hHCD)

Size :

100μg/vial

Clonality :

Monoclonal

Clone Number :

hHCD

Host :

Mouse

Reactivity :

Human, Mouse, Rabbit

Application(s) :

IHC, WB

Application Details :

Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Human, mouse, rabbit, By Heat. Western blot, 0.25-0.5µg/ml, Human, mouse, rabbit.

Application Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-Caldesmon (Smooth) Cald1 Antibody (Monoclonal, hHCD) catalog # MA1009. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rabbit.

Concentration :

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Gene Name :

CALD1

Uniprot ID :

Q62736

Immunogen :

Human uterus smooth muscle extract.

Form :

Liquid

Contents :

Each vial contains 50% glycerol, 1.2% Sodium acetate, 1% BSA, 0.02% NaN3.

Purification :

Ascites

Cross-reactivity :

No cross reactivity with other proteins

Isotype :

Mouse IgG1

Storage :

At -20˚C for one year and at 4˚C for one month from date of receipt. Avoid repeated freezing and thawing.

Gene Full Name :

Non-muscle caldesmon

Synonyms :

Non-muscle caldesmon; CDM; L-caldesmon; Cald1;

Protein Name :

Caldesmon

Molecular Weight :

60584 MW

Protein Function :

Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also play an essential role during cellular mitosis and receptor capping. Involved in Schwann cell migration during peripheral nerve regeneration.

Subcellular Localization :

Cytoplasm, cytoskeleton. Cytoplasm, myofibril. On thin filaments in smooth muscle and on stress fibers in fibroblasts (nonmuscle).

Tissue Specificity :

High-molecular-weight caldesmon (h-caldesmon) is predominantly expressed in smooth muscles, whereas low- molecular-weight caldesmon (l-caldesmon) is widely distributed in non-muscle tissues and cells. Not expressed in skeletal muscle or heart.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).

Background :

Caldesmon is a potential actomyosin regulatory protein found in smooth muscle and nonmuscle cells. The predicted smooth-muscle polypeptide is 793 amino acids long. The high molecular weight caldesmon (h-CaD) is predominantly expressed in smooth muscles, whereas the low molecular weight caldesmon (l-CaD) is widely distributed in nonmuscle tissues and cells. Hayashi et al. (1992) demonstrated that the human CDM gene is composed of 14 exons.

Research Category :

Actin Binding Proteins, Actin Etc, Cardiovascular, Contractility, Cytoskeleton, Cytoskeleton / Ecm, Microfilaments, Signal Transduction