Anti-BAP1 Antibody (Monoclonal, 3C11) | Boster MA1002 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-BAP1 Antibody (Monoclonal, 3C11)

catalog :

MA1002

quantity :

100 ug/vial

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

3C11

reactivity :

human, rat

application :

western blot

product information

sku :

MA1002

status :

Enabled

name :

Anti-BAP1 Antibody (Monoclonal, 3C11)

category name :

Primary Antibodies, Monoclonal Antibodies

gene name :

BAP1

price various sizes :

30ug sample size / $99 100ug / $240 100ug+Free HRP Secondary BA1050 / $240 100ug+Free Biotin Secondary BA1001 / $240

clonality :

Monoclonal

clone number :

3C11

concentration :

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

conjugate :

contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

description :

Monoclonal clone# G2 antibody for BAP1 detection. Host: Mouse.Size: 100ug/vial. Tested applications: WB. Reactive species: Human. BAP1 information: Molecular Weight: 80310 MW; Subcellular Localization: Cytoplasm . Nucleus . Mainly nuclear. Binds to chromatin. Localizes to the cytoplasm when monoubiquitinated by the E2/E3 hybrid ubiquitin-protein ligase UBE2O; Tissue Specificity: BAP1: Highly expressed in testis, placenta andovary. Expressed in breast.

short description :

Mouse IgG monoclonal antibody for BAP1, ubiquitin carboxy-terminal hydrolase (BAP1) detection. Tested with WB in Human. No cross reactivity with other proteins.

size :

100 ug/vial

sample size available :

30ug for $99, contact us for details

uniprot id :

D3ZHS6

host :

Mouse

immunogen :

Recombinant full-length BAP1.

form :

Lyophilized

purification :

Ascites

storage :

At -20 C for one year. After reconstitution, at 4 C for one month. It can also be aliquotted and stored frozen at -20 C for a longer time.Avoid repeated freezing and thawing.

cross reactivity :

No cross reactivity with other proteins

isotype :

Mouse IgG1

reconstitution :

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

application details :

Western blot, 0.5ug/ml, Human.

applications :

reactivity :

Human

image labels :

Anti-BAP1 antibody (monoclonal), MA1002, Western blotting. Lane: MCF-7 Cell Lysate

background :

BAP1, also known as BRCA1-associated protein-1, contains an acidic region, a highly charged C-terminal region, and 2 putative nuclear localization signals. BAP1 is a novel ubiquitin hydrolase which binds to the BRCA1 RING finger and enhances BRCA1-mediated cell growth suppression. BAP1 is expressed as a 4-kb mRNA in all human tissues, and mapped to 3p21.3.

competitor equivalent skus :

sc 8133 sc 28236 sc 48386 sc 28383 sc 8132 sc 13576

research category :

Cancer, Cell Biology, Cell Cycle, Cell Cycle Inhibitors, Deubiquitination, Epigenetics And Nuclear Signaling, Ubiquitin & Ubiquitin Like Modifiers

synonyms :

Ubiquitin carboxyl-terminal hydrolase BAP1;3.4.19.12;BRCA1-associated protein 1;Bap1;

gene full name :

Ubiquitin carboxyl-terminal hydrolase BAP1

molecular weight :

80310 MW

protein function :

Deubiquitinating enzyme that plays a key role in chromatin by mediating deubiquitination of histone H2A and HCFC1. Catalytic component of the PR-DUB complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-119' (H2AK119ub1). Does not deubiquitinate monoubiquitinated histone H2B. Acts as a regulator of cell growth by mediating deubiquitination of HCFC1 N-terminal and C-terminal chains, with some specificity toward 'Lys-48'- linked polyubiquitin chains compared to 'Lys-63'-linked polyubiquitin chains. Deubiquitination of HCFC1 does not lead to increase stability of HCFC1. Interferes with the BRCA1 and BARD1 heterodimer activity by inhibiting their ability to mediate ubiquitination and autoubiquitination. It however does not mediate deubiquitination of BRCA1 and BARD1. Able to mediate autodeubiquitination via intramolecular interactions to couteract monoubiquitination at the nuclear localization signal (NLS), thereby protecting it from cytoplasmic sequestration. Acts as a tumor suppressor (By similarity).

subcellular localization :

Cytoplasm . Nucleus . Mainly nuclear. Binds to chromatin. Localizes to the cytoplasm when monoubiquitinated by the E2/E3 hybrid ubiquitin-protein ligase UBE2O.

tissue specificity :

BAP1: Highly expressed in testis, placenta andovary. Expressed in breast.

protein name :

Ubiquitin carboxyl-terminal hydrolase BAP1

recommended detection systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot.

sequence similarities :

Belongs to the peptidase C12 family. BAP1 subfamily.

last modified :

1/28/19 19:39