Anti-Actin ACTA1 Antibody (Monoclonal, AC-40) | Boster MA1000 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-Actin ACTA1 Antibody (Monoclonal, AC-40)

catalog :

MA1000

quantity :

100μg/vial

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AC-40

The same clone is also sold as:

MilliporeSigma: A4700
Invitrogen: MA1-24907, MA1-24908, MA5-32995
Novus Biologicals: NB120-11003

reactivity :

human, mouse, rat, chicken

application :

western blot, immunohistochemistry

product information

SKU :

MA1000

Product Name :

Anti-Actin ACTA1 Antibody (Monoclonal, AC-40)

Size :

100μg/vial

Clonality :

Monoclonal

Clone Number :

AC-40

Host :

Mouse

Reactivity :

Chicken, Human, Mouse, Rat

Predicted Reactivity :

Chicken

Application(s) :

IHC, WB

Application Details :

Immunohistochemistry (Frozen Section), 4µg/ml, Human, chicken, mouse, rat Western blot, 2µg/ml, Human, chicken, mouse, rat.

Application Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-Actin ACTA1 Antibody (Monoclonal, AC-40) catalog # MA1000. Tested in IHC, WB applications. This antibody reacts with Chicken, Human, Mouse, Rat.

Concentration :

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Gene Name :

Actin

Uniprot ID :

P68133

Immunogen :

Synthetic actin C-terminal peptideSer-Gly-Pro-Ser-Ile-Val-His-Arg-Lys-Cys-Phe,attached to a Multiple Antigen Peptide (MAP) backbone.

Form :

Lyophilized

Contents :

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Purification :

Ascites

Cross-reactivity :

No cross reactivity with other proteins

Isotype :

Mouse IgG2a

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Gene Full Name :

Actin, alpha skeletal muscle

Synonyms :

Actin, alpha skeletal muscle; Alpha-actin-1; ACTA1; ACTA;

Protein Name :

Actin, alpha skeletal muscle Actin, alpha cardiac muscle 1 Actin, aortic smooth muscle Actin, cytoplasmic 1 Actin, cytoplasmic 2 Actin, gamma-enteric smooth muscle

Molecular Weight :

42051 MW

Protein Function :

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Subcellular Localization :

Cytoplasm, cytoskeleton.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F).

Background :

Actin, a highly conserved protein, is a major component of both the cytoskeletal and contractile structures in the cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. The actins exhibit over 90% sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three alpha-actin, one beta-actin, two gamma-actin. Because the amino acid sequence of the C-terminal is the same for almost all actins, this antibody has been raised using a synthetic peptide corresponding to the C-terminal 11 residues.

Research Category :

Actin, Actin Assembly, Actin Binding Proteins, Actin Etc, Apoptosis, Cancer, Cell Death, Cytoskeleton, Cytoskeleton / Ecm, Invasion/Microenvironment, Microfilaments, Receptors, Signal Transduction, Subcellular Markers, Tags & Cell Markers