Anti-CRISPR-Cas9 Rabbit Monoclonal Antibody | Boster M30929-1 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-CRISPR-Cas9 Rabbit Monoclonal Antibody

catalog :

M30929-1

quantity :

100 µl

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

ABCH-3

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry

product information

SKU :

M30929-1

Product Name :

Anti-CRISPR-Cas9 Rabbit Monoclonal Antibody

Size :

100 µl

Clonality :

Monoclonal

Clone Number :

ABCH-3

Host :

Rabbit

Reactivity :

Streptococcus pyogenes

Application(s) :

Flow Cytometry, IP, IF, IHC, ICC, WB

Application Details :

WB 1:1000-1:5000 .IHC 1:50-1:200 .ICC/IF 1:50-1:200 .IP 1:30 .Flow Cytometry 1:50

Description :

Boster Bio Anti-CRISPR-Cas9 Rabbit Monoclonal Antibody catalog # M30929-1. Tested in Flow Cytometry, IP, IF, IHC, ICC, WB applications. This antibody reacts with Streptococcus pyogenes.

Concentration :

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Gene Name :

cas9

Uniprot ID :

J7RUA5

Immunogen :

A synthesized peptide derived from human CRISPR-Cas9

Form :

Liquid

Contents :

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Purification :

Affinity-chromatography

Storage :

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Gene Full Name :

CRISPR-associated endonuclease Cas9

Synonyms :

CRISPR-associated endonuclease Cas9 ; 3.1.-.- ; SaCas9 ; cas9 ;

Molecular Weight :

123949 MW

Protein Function :

CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer; Cas9 is inactive in the absence of the 2 guide RNAs (gRNA). Cas9 recognizes the protospacer adjacent motif (PAM) in the CRISPR repeat sequences to help distinguish self versus nonself, as targets within the bacterial CRISPR locus do not have PAMs. PAM recognition is also required for catalytic activity.