Anti-CCT3 Antibody Picoband™ (monoclonal, 12H4) | Boster M05920 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-CCT3 Antibody Picoband™ (monoclonal, 12H4)

catalog :

M05920

quantity :

100µg/vial

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

12H4

The same clone is also sold as:

Invitrogen: MA5-27872

reactivity :

human

application :

western blot, immunocytochemistry

product information

SKU :

M05920

Product Name :

Anti-CCT3 Antibody Picoband™ (monoclonal, 12H4)

Size :

100µg/vial

Clonality :

Monoclonal

Clone Number :

12H4

Host :

Mouse

Reactivity :

Human

Application(s) :

IF, ICC, WB

Application Details :

Western blot, 0.1-0.5µg/ml. Immunocytochemistry/Immunofluorescence, 5µg/ml

Description :

Boster Bio Anti-CCT3 Antibody Picoband™ (monoclonal, 12H4) catalog # M05920. Tested in IF, ICC, WB applications. This antibody reacts with Human.

Concentration :

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Gene Name :

CCT3

Uniprot ID :

P49368

Immunogen :

different from the related mouse and rat sequences by one amino acid.
EPLAVKLQTYKTAVETAVLLLRIDDIVSGHKKKGDDQSR
Q),
A synthetic peptide corresponding to a sequence at the C-terminus of human CCT3 (497-536aa

Form :

Lyophilized

Contents :

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Cross-reactivity :

No cross reactivity with other proteins.

Isotype :

IgG1

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Gene Full Name :

chaperonin containing TCP1 subunit 3

Synonyms :

T-complex protein 1 subunit gamma; TCP-1-gamma; CCT-gamma; hTRiC5; CCT3; CCTG; TRIC5

Protein Function :

Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.

Subcellular Localization :

Cytoplasm

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti- Mouse IgG Super Vision Assay Kit (SV0001-1) for ICC.

Background :

T-complex protein 1 subunit gamma is a protein that in humans is encoded by the CCT3 gene. The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants have been characterized for this gene. In addition, a pseudogene of this gene has been found on chromosome 8.

Research Category :

Chaperones, Protein Trafficking, Signal Transduction