Anti-Myogenin (Skeletal Muscle Marker) Monoclonal Antibody

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-Myogenin (Skeletal Muscle Marker) Monoclonal Antibody

catalog :

M03238

quantity :

100 ug/vial

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

F5D

reactivity :

human, mouse, rat, dogs

application :

immunohistochemistry, immunoprecipitation

product information

sku :

M03238

status :

Enabled

name :

Anti-Myogenin (Skeletal Muscle Marker) Monoclonal Antibody

category name :

Primary Antibodies, Monoclonal Antibodies, IHC ICC IF Antibodies

gene name :

MYOG

price various sizes :

20ug sample size / $99 100ug / $299 100ug+Free HRP Secondary BA1050 / $299 100ug+Free Biotin Secondary BA1001 / $299

clonality :

Monoclonal

clone number :

F5D

concentration :

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

conjugate :

contents :

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

description :

Mouse Monoclonal Antibody [Clone F5D] antibody for Myogenin/MYOG detection. Host: Mouse.Size: 100ug/vial. Tested applications: IP. Reactive species: Canine. Myogenin/MYOG information: Molecular Weight: 25037 MW; Subcellular Localization: Nucleus. Recruited to late myogenic gene promoter regulatory sequences with SMARCA4/BRG1/BAF190A and SWI/SNF chromatin-remodeling enzymes to promote chromatin- remodeling and transcription initiation in developing embryos.

short description :

Anti-Myogenin (Skeletal Muscle Marker) Antibody

size :

100 ug/vial

sample size available :

20ug for $99, contact us for details

uniprot id :

P15173

host :

Mouse

immunogen :

Rat myogenin peptide (aa 73-94) followed by rat myogenin recombinant fragment (aa30-224) (Epitope aa138-158)

form :

Liquid

application details :

Immunoprecipitation (0.5-1ug/500ug protein Lysate). Immunohistology (Formalin-fixed) (0.5-1.0ug/ml for 30 minutes at RT). Immunofluorescence (0.5-1ug/ml)

applications :

IP, IHC

reactivity :

Canine, Human, Mouse, Pig, Rat

image labels :

Formalin-fixed, paraffin-embedded human Rhabdomyosarcoma stained withMyogenin Monoclonal Antibody (F5D)

research category :

Mesenchymal Stem Cells, Stem Cells, Transcription

synonyms :

Myogenin;Class C basic helix-loop-helix protein 3;bHLHc3;Myogenic factor 4;Myf-4;MYOG;BHLHC3, MYF4;

gene full name :

Myogenin

molecular weight :

25037 MW

protein function :

Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation, cell cycle exit and muscle atrophy. Essential for the development of functional embryonic skeletal fiber muscle differentiation. However is dispensable for postnatal skeletal muscle growth; phosphorylation by CAMK2G inhibits its transcriptional activity in respons to muscle activity. Required for the recruitment of the FACT complex to muscle-specific promoter regions, thus promoting gene expression initiation. During terminal myoblast differentiation, plays a role as a strong activator of transcription at loci with an open chromatin structure previously initiated by MYOD1. Together with MYF5 and MYOD1, co-occupies muscle-specific gene promoter core regions during myogenesis. Cooperates also with myocyte-specific enhancer factor MEF2D and BRG1-dependent recruitment of SWI/SNF chromatin- remodeling enzymes to alter chromatin structure at myogenic late gene promoters. Facilitates cell cycle exit during terminal muscle differentiation through the up-regulation of miR-20a expression, which in turn represses genes involved in cell cycle progression. Binds to the E-box containing (E1) promoter region of the miR-20a gene. Plays also a role in preventing reversal of muscle cell differentiation. Contributes to the atrophy-related gene expression in adult denervated muscles. Induces fibroblasts to differentiate into myoblasts (By similarity).

subcellular localization :

Nucleus. Recruited to late myogenic gene promoter regulatory sequences with SMARCA4/BRG1/BAF190A and SWI/SNF chromatin-remodeling enzymes to promote chromatin- remodeling and transcription initiation in developing embryos.

last modified :

2/6/19 22:57