Anti-Calbindin Antibody | Boster M03047-2 product information

product summary

company name :

Boster

product type :

antibody

product name :

Anti-Calbindin Antibody

catalog :

M03047-2

quantity :

100 ul

price :

299 USD

clonality :

polyclonal

host :

chicken

conjugate :

nonconjugated

reactivity :

human, mouse, rat, bovine

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Boster product webpage

citations: 1

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; mouse; loading ...; fig 1e	Joshi D, Zhang C, Babujee L, Vevea J, August B, Sheng Z, et al. Inappropriate Intrusion of an Axonal Mitochondrial Anchor into Dendrites Causes Neurodegeneration. Cell Rep. 2019;29:685-696.e5 pubmed publisher

image

image 1 :

Western blot analysis of different tissue lysates and recombinant protein solutions using chicken pAb to calbindin, M03047-2, dilution 1:5,000 in green: [1] protein standard (red), [2] rat cerebellum, [3] pig hippocampus, [4] cow cerebellum, [5] protein standard (red). Next lanes are full length recombinant human proteins, [6] Parvalbumin, [7] Calretinin, [8] Calbindin. Bands at 25kDa in tissue lysates and ~30 kDa in protein solutions correspond to calbindin, the recombinant form being slightly larger due to the presence of a His tag and other vector derived sequence. The M03047-2 antibody specifically recognizes calbindin protein and does not react with the closely related proteins parvalbumin and calretinin.

image 2 :

Immunofluorescent analysis of rat cerebellum section stained with chicken pAb to calbindin, M03047-2, dilution 1:2,000, in green, and costained with rabbit pAb to MeCP2, dilution 1:5,000, in red. Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45?M, and free-floating sections were stained with above antibodies. Calbindin, often used as a Purkinje cell marker, is prominently detected in dendrites and perikarya of these cells in the cerebellar molecular layer. The MeCP2 antibody selectively stains nuclei of neuronal cells.

product information

SKU :

M03047-2

Product Name :

Anti-Calbindin Antibody

Price :

299 USD

Size :

100 ul

Clonality :

Polyclonal

Host :

Chicken

Reactivity :

Human, Bovine, Rat, Mouse

Application(s) :

IF, IHC, WB

Application Details :

WB: 1:1,000-1:5,000. IF/ICC or IHC: 1:1,000-1:5,000.

Description :

Boster Bio Anti-Calbindin Antibody catalog # M03047-2. Tested in IF, IHC, WB applications. This antibody reacts with Bovine, Human, Mouse, Rat.

Concentration :

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Gene Name :

CALB1

Uniprot ID :

P05937

Immunogen :

Full-length recombinant human protein

Contents :

Supplied as an aliquot of IgY preparation plus 5mM NaN 3

Storage :

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Gene Full Name :

Calbindin

Synonyms :

Calbindin; Calbindin D28; D-28K; Vitamin D-dependent calcium-binding protein, avian-type; CALB1; CAB27;

Molecular Weight :

30025 MW

Protein Function :

Buffers cytosolic calcium. May stimulate a membrane Ca (2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase.

Research Category :

Calcium Binding Proteins, Calcium Signaling, Cell Type Marker, Neuron Marker, Neuroscience, Neurotransmission

more info or order :

Boster product webpage