Anti-CDC34/Ubch3 Rabbit Monoclonal Antibody | Boster M03038 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-CDC34/Ubch3 Rabbit Monoclonal Antibody

catalog :

M03038

quantity :

100 µl

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

IBO-3

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, immunoprecipitation, flow cytometry

product information

SKU :

M03038

Product Name :

Anti-CDC34/Ubch3 Rabbit Monoclonal Antibody

Size :

100 µl

Clonality :

Monoclonal

Clone Number :

IBO-3

Host :

Rabbit

Reactivity :

Human, Mouse, Rat

Application(s) :

Flow Cytometry, IP, IF, ICC, WB

Application Details :

WB 1:500-1:2000 .ICC/IF 1:50-1:200 .IP 1:50 .Flow Cytometry 1:50

Description :

Boster Bio Anti-CDC34/Ubch3 Rabbit Monoclonal Antibody catalog # M03038. Tested in Flow Cytometry, IP, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Concentration :

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Gene Name :

CDC34

Uniprot ID :

P49427

Immunogen :

A synthesized peptide derived from human CDC34

Form :

Liquid

Contents :

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Purification :

Affinity-chromatography

Storage :

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Gene Full Name :

Ubiquitin-conjugating enzyme E2 R1

Synonyms :

Ubiquitin-conjugating enzyme E2 R1; 6.3.2.19; Ubiquitin-conjugating enzyme E2-32 kDa complementing; Ubiquitin-conjugating enzyme E2-CDC34; Ubiquitin-protein ligase R1; CDC34; UBCH3, UBE2R1;

Molecular Weight :

26737 MW

Protein Function :

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys- 48'-linked polyubiquitination. Cooperates with the E2 UBCH5C and the SCF (FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation. Performs ubiquitin chain elongation building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. UBE2D3 acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin. Cooperates with the SCF (SKP2) E3 ligase complex to regulate cell proliferation through ubiquitination and degradation of MYBL2 and KIP1. Involved in ubiquitin conjugation and degradation of CREM isoform ICERIIgamma and ATF15 resulting in abrogation of ICERIIgamma- and ATF5- mediated repression of cAMP-induced transcription during both meiotic and mitotic cell cycles. Involved in the regulation of the cell cycle G2/M phase through its targeting of the WEE1 kinase for ubiquitination and degradation. Also involved in the degradation of beta-catenin. Is target of human herpes virus 1 protein ICP0, leading to ICP0-dependent dynamic interaction with proteasomes.

Subcellular Localization :

Cytoplasm. Nucleus. The phosphorylation of the C-terminal tail plays an important role in mediating nuclear localization. Colocalizes with beta-tubulin on mitotic spindles in anaphase.

Tissue Specificity :

Expressed in testes during spermatogenesis to regulate repression of cAMP-induced transcription.

Research Category :

Chromatin Modifying Enzymes, Epigenetics and Nuclear Signaling, Ubiquitin & Ubiquitin Like Modifiers