Anti-RAB7 RAB7A Rabbit Monoclonal Antibody | Boster M02409 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-RAB7 RAB7A Rabbit Monoclonal Antibody

catalog :

M02409

quantity :

100 µl

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

GHA-18

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry

product information

SKU :

M02409

Product Name :

Anti-RAB7 RAB7A Rabbit Monoclonal Antibody

Size :

100 µl

Clonality :

Monoclonal

Clone Number :

GHA-18

Host :

Rabbit

Reactivity :

Human, Mouse, Rat

Application(s) :

Flow Cytometry, IF, IHC, ICC, WB

Application Details :

WB 1:1000-1:2000 .IHC 1:50-1:200 .ICC/IF 1:50-1:200 .Flow Cytometry 1:50

Description :

Boster Bio Anti-RAB7 RAB7A Rabbit Monoclonal Antibody catalog # M02409. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Concentration :

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Gene Name :

RAB7A

Uniprot ID :

P51149

Immunogen :

A synthesized peptide derived from human RAB7

Form :

Liquid

Contents :

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Purification :

Affinity-chromatography

Storage :

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Gene Full Name :

Ras-related protein Rab-7a

Synonyms :

Ras-related protein Rab-7a; RAB7A; RAB7;

Molecular Weight :

23490 MW

Protein Function :

Key regulator in endo-lysosomal trafficking. Governs early-to-late endosomal maturation, microtubule minus-end as well as plus-end directed endosomal migration and positioning, and endosome-lysosome transport through different protein-protein interaction cascades. Plays a central role, not only in endosomal traffic, but also in many other cellular and physiological events, such as growth-factor-mediated cell signaling, nutrient- transportor mediated nutrient uptake, neurotrophin transport in the axons of neurons and lipid metabolism. Also involved in regulation of some specialized endosomal membrane trafficking, such as maturation of melanosomes, pathogen-induced phagosomes (or vacuoles) and autophagosomes. Plays a role in the maturation and acidification of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis. Plays a role in the fusion of phagosomes with lysosomes. Plays important roles in microbial pathogen infection and survival, as well as in participating in the life cycle of viruses. Microbial pathogens possess survival strategies governed by RAB7A, sometimes by employing RAB7A function (e.g. Salmonella) and sometimes by excluding RAB7A function (e.g. Mycobacterium). In concert with RAC1, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. Controls the endosomal trafficking and neurite outgrowth signaling of NTRK1/TRKA (PubMed:11179213, PubMed:12944476, PubMed:14617358, PubMed:20028791, PubMed:21255211). Regulates the endocytic trafficking of the EGF- EGFR complex by regulating its lysosomal degradation. Involved in the ADRB2-stimulated lipolysis through lipophagy, a cytosolic lipase-independent autophagic pathway (By similarity). Required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413).

Subcellular Localization :

Cytoplasmic vesicle, phagosome membrane ; Peripheral membrane protein ; Cytoplasmic side. Late endosome membrane ; Peripheral membrane protein ; Cytoplasmic side. Lysosome membrane ; Peripheral membrane protein ; Cytoplasmic side. Melanosome membrane ; Peripheral membrane protein ; Cytoplasmic side. Cytoplasmic vesicle, autophagosome membrane ; Peripheral membrane protein ; Cytoplasmic side. Lipid droplet. Colocalizes with OSBPL1A at the late endosome (PubMed:16176980). Found in the ruffled border (a late endosomal-like compartment in the plasma membrane) of bone-resorbing osteoclasts. Recruited to phagosomes containing S.aureus or Mycobacterium (PubMed:21255211). Lipid droplet localization is increased upon ADRB2 stimulation (By similarity).

Tissue Specificity :

Widely expressed; high expression found in skeletal muscle.

Research Category :

G Protein Signaling, Organelles, Protein Trafficking, Ras Family, Signal Transduction, Signaling Pathway, Small G Proteins, Subcellular Markers, Vesicle Transport