Anti-ATP citrate lyase ACLY Antibody Picoband™ (monoclonal, 5I2)

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-ATP citrate lyase ACLY Antibody Picoband™ (monoclonal, 5I2)

catalog :

M02372-1

quantity :

100µg/vial

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

5i2

The same clone is also sold as:

Invitrogen: MA5-49221

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

product information

SKU :

M02372-1

Product Name :

Anti-ATP citrate lyase ACLY Antibody Picoband™ (monoclonal, 5I2)

Size :

100µg/vial

Clonality :

Monoclonal

Clone Number :

5I2

Host :

Mouse

Reactivity :

Human, Mouse, Rat

Application(s) :

Flow Cytometry, IF, IHC-P, ICC, WB

Application Details :

Western blot, 0.1-0.5µg/ml. Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml. Immunocytochemistry/Immunofluorescence, 2µg/ml, Human. Flow Cytometry, 1-3µg/1x10^6 cells.

Description :

Boster Bio Anti-ATP citrate lyase ACLY Antibody Picoband™ (monoclonal, 5I2) catalog # M02372-1. Tested in Flow Cytometry, IF, IHC-P, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Concentration :

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Gene Name :

ACLY

Uniprot ID :

P53396

Immunogen :

E. coli-derived human ATP citrate lyase recombinant protein (Position: M1-I180). Human ATP citrate lyase shares 95% amino acid (aa) sequence identity with both mouse and rat ATP citrate lyase.

Form :

Lyophilized

Contents :

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Cross-reactivity :

No cross reactivity with other proteins.

Isotype :

IgG2b

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500µg/ml.

Gene Full Name :

ATP citrate lyase

Synonyms :

ATP-citrate synthase; ATP-citrate (pro-S-)-lyase; ACL; Citrate cleavage enzyme; ACLY

Protein Function :

ATP-citrate synthase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine.

Subcellular Localization :

Cytoplasm

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.

Background :

ATP citrate lyase, aslo known as ACLY, is an enzyme that in animals represents an important step in fatty acid biosynthesis. ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer of apparently identical subunits. The product, acetyl-CoA, in animals serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. It is activated by insulin. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine.?In plants, ATP citrate lyase generates the acetyl-CoA for cytosolically-synthesized metabolites.

Research Category :

Cancer, Cancer Metabolism, Energy Metabolism, Energy Transfer Pathways, Integration Of Energy, Integration Of Energy Metabolism, Lipid Metabolism, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Pathways And Processes, Signal Transduction