Anti-Rt CD8a Purified Monoclonal Antibody | Boster M02236-3 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-Rt CD8a Purified Monoclonal Antibody

catalog :

M02236-3

quantity :

0.1 mg (C100)

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

OX-8

The same clone is also sold as:

Bio-Rad: MCA48A488, MCA48PE, MCA48GA, MCA48P647, MCA48A647, MCA48G, MCA48R
BioLegend: 201706, 201710, 201701, 201705, 201716, 201712, 201715, 201703
Santa Cruz Biotechnology: sc-53063
BD Biosciences: 562180, 554855, 550298, 554857, 561614, 554854, 559976, 558824
Invitrogen: 63-0084-82, MA5-17395, MA5-17396, 14-0084-82, MA5-17398, 56-0084-82, 46-0084-80, MA5-44018, 25-0084-82, MA5-44075, MA5-17400, 78-0084-80, 62-0084-82, MA1-70003, 17-0084-82, MA1-80658, 48-0084-80, 48-0084-82, MA5-17394, 78-0084-82, MA1-81595, MA5-17399, 46-0084-82, 12-0084-82, 11-0084-82, MA5-17397, MA5-17401, 14-0084-37, 56-0084-80
Novus Biologicals: NBP2-12523
Abcam: ab33786
Exbio: 11-678-C100, 11-678-C025
US Biological: C2258-90M
Absolute Antibody: Ab00544-8.1, Ab00544-1.1, Ab00544-23.0, Ab00544-8.4
OriGene: SM259F, SM259FX, SM259R, TA363862, SM259PX, SM259PS, SM259P, SM259FS
MyBioSource: MBS520223, MBS520546

reactivity :

rat

application :

western blot, immunohistochemistry, immunoprecipitation, flow cytometry

product information

SKU :

M02236-3

Product Name :

Anti-Rt CD8a Purified Monoclonal Antibody

Size :

0.1 mg (C100)

Clonality :

Monoclonal

Clone Number :

OX-8

Host :

Mouse

Reactivity :

Rat

Application(s) :

Flow Cytometry, IP, WB, IHC

Application Notes :

Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-Rt CD8a Purified Monoclonal Antibody catalog # M02236-3. Tested in Flow Cytometry, IP, IHC, WB applications. This antibody reacts with Rat.

Concentration :

1 mg/ml

Gene Name :

Cd8a

Uniprot ID :

P07725

Immunogen :

High Mw glycoproteins from rat thymocytes

Form :

Liquid

Contents :

Phosphate buffered saline (PBS) solution with 15 mM sodium azide

Purification :

Purified by protein-A affinity chromatography

Isotype :

Mouse IgG1 kappa

Storage :

Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label.

Gene Full Name :

vacuolar protein sorting 4 homolog A

Synonyms :

Vacuolar protein sorting-associated protein 4A; Protein SKD2; VPS4-1; hVPS4; VPS4A; VPS4

Protein Function :

Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). Involved in cytokinesis: retained at the midbody by ZFYVE19/ANCHR and CHMP4C until abscission checkpoint signaling is terminated at late cytokinesis. It is then released following dephosphorylation of CHMP4C, leading to abscission (PubMed:24814515). VPS4A/B are required for the exosomal release of SDCBP, CD63 and syndecan.

Subcellular Localization :

Late endosome membrane. Peripheral membrane protein. Prevacuolar compartment membrane. Midbody.

Tissue Specificity :

Ubiquitously expressed.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Background :

Vacuolar protein sorting-associated protein 4A is a protein that in humans is encoded by the VPS4A gene. It is mapped to 16q22.1. The protein encoded by this gene is a member of the AAA protein family (ATPases associated with diverse cellular activities), and is the homolog of the yeast Vps4 protein. In humans, two paralogs of the yeast protein have been identified. The former share a high degree of aa sequence similarity with each other, and also with yeast Vps4 and mouse Skd1 proteins. The mouse Skd1 (suppressor of K+ transport defect 1) has been shown to be really an yeast Vps4 ortholog. Functional studies indicate that both human paralogs associate with the endosomal compartments, and are involved in intracellular protein trafficking, similar to Vps4 protein in yeast. The gene encoding this paralog has been mapped to chromosome 16; the gene for the other resides on chromosome 18.