antibody

Anti-PKC beta 2 PRKCB Rabbit Monoclonal Antibody

M01940

100 µl

monoclonal

domestic rabbit

nonconjugated

DOC-16

human, mouse, rat

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

Anti-PKC beta 2 PRKCB Rabbit Monoclonal Antibody

100 µl

Monoclonal

DOC-16

Rabbit

Human, Mouse, Rat

IP, IF, IHC, ICC, WB

WB 1:500-1:2000 .IHC 1:50-1:200 .ICC/IF 1:50-1:200 .IP 1:50

Boster Bio Anti-PKC beta 2 PRKCB Rabbit Monoclonal Antibody catalog # M01940. Tested in IP, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

PRKCB

P05771

A synthesized peptide derived from human PKC beta 2

Liquid

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Affinity-chromatography

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Protein kinase C beta type

Protein kinase C beta type; PKC-B; PKC-beta; 2.7.11.13; PRKCB; PKCB, PRKCB1;

76869 MW

Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase involved in various cellular processes such as regulation of the B-cell receptor (BCR) signalosome, oxidative stress-induced apoptosis, androgen receptor-dependent transcription regulation, insulin signaling and endothelial cells proliferation. Plays a key role in B-cell activation by regulating BCR-induced NF-kappa-B activation. Mediates the activation of the canonical NF-kappa-B pathway (NFKB1) by direct phosphorylation of CARD11/CARMA1 at 'Ser-559', 'Ser-644' and 'Ser-652'. Phosphorylation induces CARD11/CARMA1 association with lipid rafts and recruitment of the BCL10-MALT1 complex as well as MAP3K7/TAK1, which then activates IKK complex, resulting in nuclear translocation and activation of NFKB1. Plays a direct role in the negative feedback regulation of the BCR signaling, by down-modulating BTK function via direct phosphorylation of BTK at 'Ser-180', which results in the alteration of BTK plasma membrane localization and in turn inhibition of BTK activity. Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates 'Ser-36' of isoform p66Shc of SHC1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 'Lys-4' (H3K4me) by LSD1/KDM1A. In insulin signaling, may function downstream of IRS1 in muscle cells and mediate insulin-dependent DNA synthesis through the RAF1- MAPK/ERK signaling cascade. May participate in the regulation of glucose transport in adipocytes by negatively modulating the insulin-stimulated translocation of the glucose transporter SLC2A4/GLUT4. Under high glucose in pancreatic beta-cells, is probably involved in the inhibition of the insulin gene transcription, via regulation of MYC expression. In endothelial cells, activation of PRKCB induces increased phosphorylation of RB1, increased VEGFA-induced cell proliferation, and inhibits PI3K/AKT-dependent nitric oxide synthase (NOS3/eNOS) regulation by insulin, which causes endothelial dysfunction. Also involved in triglyceride homeostasis (By similarity). Phosphorylates ATF2 which promotes cooperation between ATF2 and JUN, activating transcription.

Cytoplasm. Nucleus. Membrane ; Peripheral membrane protein.

Domain Families, Epigenetics and Nuclear Signaling, Protein Phosphorylation, Receptor Tyrosine Kinases, Signal Transduction, Transcription, Transcription Factors, Tyrosine Kinases, Zinc Finger