Anti-Ms CD90.2 Purified Azide Free Thy1 Monoclonal Antibody

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-Ms CD90.2 Purified Azide Free Thy1 Monoclonal Antibody

catalog :

M01818-2

quantity :

0.1 mg (C100)

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1aG4/C5

The same clone is also sold as:

Novus Biologicals: NBP1-44877
Exbio: 1P-443-C100, 1P-443-C025, 10-443-C100, 10-443-C025
OriGene: SM3158A, SM3158R
Invitrogen: MA1-19029

reactivity :

mouse

application :

western blot, immunocytochemistry, flow cytometry

product information

SKU :

M01818-2

Product Name :

Anti-Ms CD90.2 Purified Azide Free Thy1 Monoclonal Antibody

Size :

0.1 mg (C100)

Clonality :

Monoclonal

Clone Number :

1aG4/C5

Host :

Mouse

Reactivity :

Mouse

Application(s) :

Flow Cytometry, WB, ICC

Application Notes :

Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-Ms CD90.2 Purified Azide Free Thy1 Monoclonal Antibody catalog # M01818-2. Tested in Flow Cytometry, ICC, WB applications. This antibody reacts with Mouse.

Concentration :

1 mg/ml

Gene Name :

Thy1

Uniprot ID :

P01831

Immunogen :

thymocytes from C3H/Di mice (Thy-1.2 positive)

Form :

Liquid

Contents :

Azide free phosphate buffered saline (PBS), approx. pH 7.4; 0.2 μm filter sterilized

Purification :

Purified by protein-A affinity chromatography

Isotype :

Mouse IgG3

Storage :

Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label.

Gene Full Name :

protein inhibitor of activated STAT 1

Synonyms :

E3 SUMO-protein ligase PIAS1; DEAD/H box-binding protein 1; E3 SUMO-protein transferase PIAS1; Gu-binding protein; GBP; Protein inhibitor of activated STAT protein 1; RNA helicase II-binding protein; PIAS1; DDXBP1

Protein Function :

Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. In vitro, binds A/T-rich DNA. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context. Sumoylates PML (at'Lys-65' and 'Lys-160') and PML-RAR and promotes their ubiquitin-mediated degradation. PIAS1-mediated sumoylation of PML promotes its interaction with CSNK2A1/CK2 which in turn promotes PML phosphorylation and degradation. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation. Plays a dynamic role in adipogenesis by promoting the SUMOylation and degradation of CEBPB.

Subcellular Localization :

Nucleus speckle. PML body.

Tissue Specificity :

Expressed in numerous tissues with highest level in testis.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for ICC.

Background :

E3 SUMO-protein ligase PIAS1 is an enzyme that in humans is encoded by the PIAS1 gene. It is mapped to 15q23. This gene encodes a member of the protein inhibitor of activated STAT (PIAS) family. PIAS proteins function as SUMO E3 ligases and play important roles in many cellular processes by mediating the sumoylation of target proteins. This protein plays a central role as a transcriptional coregulator of numerous cellular pathways includign the STAT1 and nuclear factor kappaB pathways. Alternate splicing results in multiple transcript variants.