Anti-S6K1 RPS6KB1 Rabbit Monoclonal Antibody | Boster M01475-1 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-S6K1 RPS6KB1 Rabbit Monoclonal Antibody

catalog :

M01475-1

quantity :

100 µl

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

CDB-18

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation

product information

SKU :

M01475-1

Product Name :

Anti-S6K1 RPS6KB1 Rabbit Monoclonal Antibody

Size :

100 µl

Clonality :

Monoclonal

Clone Number :

CDB-18

Host :

Rabbit

Reactivity :

Human, Mouse, Rat

Application(s) :

IP, WB

Application Details :

WB 1:500-1:2000 .IHC 1:50-1:200 . IP 1:50

Description :

Boster Bio Anti-S6K1 RPS6KB1 Rabbit Monoclonal Antibody catalog # M01475-1. Tested in IP, WB applications. This antibody reacts with Human, Mouse, Rat.

Concentration :

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Gene Name :

RPS6KB1

Uniprot ID :

P23443

Immunogen :

A synthesized peptide derived from human S6K1

Form :

Liquid

Contents :

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Purification :

Affinity-chromatography

Storage :

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Gene Full Name :

Ribosomal protein S6 kinase beta-1

Synonyms :

Ribosomal protein S6 kinase beta-1; S6K-beta-1; S6K1; 2.7.11.1; 70 kDa ribosomal protein S6 kinase 1; P70S6K1; p70-S6K 1; Ribosomal protein S6 kinase I; Serine/threonine-protein kinase 14A; p70 ribosomal S6 kinase alpha; p70 S6 kinase alpha; p70 S6K-alpha; p70 S6KA; RPS6KB1; STK14A;

Molecular Weight :

59140 MW

Protein Function :

Serine/threonine-protein kinase that acts downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression. Regulates protein synthesis through phosphorylation of EIF4B, RPS6 and EEF2K, and contributes to cell survival by repressing the pro-apoptotic function of BAD. Under conditions of nutrient depletion, the inactive form associates with the EIF3 translation initiation complex. Upon mitogenic stimulation, phosphorylation by the mammalian target of rapamycin complex 1 (mTORC1) leads to dissociation from the EIF3 complex and activation. The active form then phosphorylates and activates several substrates in the pre-initiation complex, including the EIF2B complex and the cap-binding complex component EIF4B. Also controls translation initiation by phosphorylating a negative regulator of EIF4A, PDCD4, targeting it for ubiquitination and subsequent proteolysis. Promotes initiation of the pioneer round of protein synthesis by phosphorylating POLDIP3/SKAR. In response to IGF1, activates translation elongation by phosphorylating EEF2 kinase (EEF2K), which leads to its inhibition and thus activation of EEF2. Also plays a role in feedback regulation of mTORC2 by mTORC1 by phosphorylating RICTOR, resulting in the inhibition of mTORC2 and AKT1 signaling. Mediates cell survival by phosphorylating the pro-apoptotic protein BAD and suppressing its pro-apoptotic function. Phosphorylates mitochondrial URI1 leading to dissociation of a URI1-PPP1CC complex. The free mitochondrial PPP1CC can then dephosphorylate RPS6KB1 at Thr-412, which is proposed to be a negative feedback mechanism for the RPS6KB1 anti- apoptotic function. Mediates TNF-alpha-induced insulin resistance by phosphorylating IRS1 at multiple serine residues, resulting in accelerated degradation of IRS1. In cells lacking functional TSC1- 2 complex, constitutively phosphorylates and inhibits GSK3B. May be involved in cytoskeletal rearrangement through binding to neurabin. Phosphorylates and activates the pyrimidine biosynthesis enzyme CAD, downstream of MTOR.

Subcellular Localization :

Cell junction, synapse, synaptosome. Mitochondrion outer membrane. Mitochondrion. Colocalizes with URI1 at mitochondrion.

Tissue Specificity :

Widely expressed.

Research Category :

Cell Biology, Cell Cycle, Cell Cycle Inhibitors, DNA / RNA, DNA Damage Response, Epigenetics and Nuclear Signaling, Metabolism, Obesity, Protein Phosphorylation, Ribosome, Ser / Thr Kinases, Signal Transduction, Translation