Anti-Raptor RPTOR Rabbit Monoclonal Antibody | Boster M01463 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-Raptor RPTOR Rabbit Monoclonal Antibody

catalog :

M01463

quantity :

100 µl

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

EGB-18

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry

product information

SKU :

M01463

Product Name :

Anti-Raptor RPTOR Rabbit Monoclonal Antibody

Size :

100 µl

Clonality :

Monoclonal

Clone Number :

EGB-18

Host :

Rabbit

Reactivity :

Human, Mouse, Rat

Application(s) :

Flow Cytometry, IP, IF, IHC, ICC, WB

Application Details :

WB 1:500-1:1000 .IHC 1:50-1:100 .ICC/IF 1:50-1:100 .IP 1:30 .Flow Cytometry 1:30

Description :

Boster Bio Anti-Raptor RPTOR Rabbit Monoclonal Antibody catalog # M01463. Tested in Flow Cytometry, IP, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Concentration :

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Gene Name :

RPTOR

Uniprot ID :

Q8N122

Immunogen :

A synthesized peptide derived from human Raptor

Form :

Liquid

Contents :

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Purification :

Affinity-chromatography

Storage :

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Gene Full Name :

Regulatory-associated protein of mTOR

Synonyms :

Regulatory-associated protein of mTOR; Raptor; p150 target of rapamycin (TOR)-scaffold protein; RPTOR; KIAA1303, RAPTOR;

Molecular Weight :

149038 MW

Protein Function :

Involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1- mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Involved in ciliogenesis.

Subcellular Localization :

Cytoplasm. Lysosome. Cytoplasmic granule. Targeting to lysosomes depends on amino acid availability. In arsenite-stressed cells, accumulates in stress granules when associated with SPAG5 and association with lysosomes is drastically decreased.

Tissue Specificity :

Highly expressed in skeletal muscle, and in a lesser extent in brain, lung, small intestine, kidney and placenta. Isoform 3 is widely expressed, with highest levels in nasal mucosa and pituitary and lowest in spleen.

Research Category :

Adapters, Cell Biology, Cell Cycle, Cell Cycle Inhibitors, Cytoplasmic, Epigenetics and Nuclear Signaling, Signal Transduction