Anti-Collagen IV COL4A1 Antibody Picoband™ (monoclonal, 3G3)

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-Collagen IV COL4A1 Antibody Picoband™ (monoclonal, 3G3)

catalog :

M01411

quantity :

100µg/vial

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

3G3

The same clone is also sold as:

Invitrogen: MA5-36227

reactivity :

human

application :

western blot, immunocytochemistry, immunohistochemistry - paraffin section

product information

SKU :

M01411

Product Name :

Anti-Collagen IV COL4A1 Antibody Picoband™ (monoclonal, 3G3)

Size :

100µg/vial

Clonality :

Monoclonal

Clone Number :

3G3

Host :

Mouse

Reactivity :

Human

Application(s) :

IF, IHC-P, WB

Application Details :

Western blot, 0.1-0.5µg/ml. Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml. Immunofluorescence, 2µg/ml.

Description :

Boster Bio Anti-Collagen IV COL4A1 Antibody Picoband™ (monoclonal, 3G3) catalog # M01411. Tested in IF, IHC-P, WB applications. This antibody reacts with Human.

Concentration :

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Gene Name :

COL4A1

Uniprot ID :

P02462

Immunogen :

E.coli-derived human Collagen IV recombinant protein (Position: G1445-T1669). Human Collagen IV shares 97% amino acid (aa) sequence identity with mouse Collagen IV.

Form :

Lyophilized

Contents :

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Cross-reactivity :

No cross reactivity with other proteins.

Isotype :

IgG1

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Gene Full Name :

collagen, type IV, alpha 1

Synonyms :

Collagen alpha-1 (IV) chain; Arresten; COL4A1

Protein Function :

Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.

Subcellular Localization :

Secreted, extracellular space, extracellular matrix, basement membrane

Tissue Specificity :

Highly expressed in placenta.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).

Background :

COL4A1, also known as ICH or Collagen alpha-1 (IV), is a protein that in humans is encoded by the COL4A1 gene. It is mapped to 13q34. This gene encodes the major type IV alpha collagen chain of basement membranes. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. COL4A1 binds to alpha-1/beta-1 integrin and inhibits migration, proliferation, and tube formation by endothelial cells. It is also a potential therapeutic candidate for targeting tumor angiogenesis.

Research Category :

Cancer, Collagen, Cytoskeleton / Ecm, Ecm Proteins, Extracellular Matrix, Invasion/Microenvironment, Mesenchymal Stem Cells, Signal Transduction, Stem Cells