Anti-beta Actin Mouse Monoclonal Antibody | Boster M01263-4 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-beta Actin Mouse Monoclonal Antibody

catalog :

M01263-4

quantity :

100 µl/vial

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

17A10

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry

product information

SKU :

M01263-4

Product Name :

Anti-beta Actin Mouse Monoclonal Antibody

Size :

100 µl/vial

Clonality :

Monoclonal

Clone Number :

17A10

Host :

Mouse

Reactivity :

Human, Mouse, Rat, Chicken, Rabbit

Application(s) :

WB, IHC, ICC, IF

Application Details :

WB 1:3000-1:10000.IHC 1:100-1:200.ICC/IF 1:100-1:200

Application Notes :

Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-beta Actin Mouse Monoclonal Antibody catalog # M01263-4. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat, Chicken, Rabbit.

Concentration :

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Gene Name :

ACTB

Uniprot ID :

P60709

Immunogen :

A synthesized peptide derived from human beta Actin

Form :

Liquid

Contents :

Mouse IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Purification :

Affinity-chromatography

Isotype :

IgG1

Storage :

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.

Background :

Photon absorption triggers a signaling cascade in rod photoreceptors that activates cGMP phosphodiesterase (PDE), resulting in the rapid hydrolysis of cGMP, closure of cGMP-gated cation channels, and hyperpolarization of the cell. PDE is a peripheral membrane heterotrimeric enzyme made up of alpha, beta, and gamma subunits. This gene encodes the beta subunit. Mutations in this gene result in retinitis pigmentosa and autosomal dominant congenital stationary night blindness. Multiple transcript variants encoding different isoforms have been found for this gene.