Anti-Chicken PAb Microtubule Associated Protein 2 MAP2 Antibody

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product summary

company name :

Boster

product type :

antibody

product name :

Anti-Chicken PAb Microtubule Associated Protein 2 MAP2 Antibody

catalog :

M01201-3

quantity :

100 ul

clonality :

monoclonal

host :

chicken

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

product information

sku :

M01201-3

status :

Enabled

name :

Anti-Chicken PAb Microtubule Associated Protein 2 MAP2 Antibody

category name :

Primary Antibodies, Polyclonal Antibodies, IHC ICC IF Antibodies

gene name :

MAP2

clonality :

Monoclonal

concentration :

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

conjugate :

contents :

Concentrated IgY preparation, 0.02% sodium azide

description :

Anti-Chicken PAb Microtubule Associated Protein 2 MAP2 Antibody tested in WB, IF, ICC, IHC, reactive to Human, rat, mouse

short description :

Anti-Chicken PAb Microtubule Associated Protein 2 MAP2 Antibody tested in WB, IF, ICC, IHC, reactive to Human, rat, mouse

size :

100 ul

uniprot id :

P11137

host :

Chicken

immunogen :

Recombinant human construct of projection domain sequences, amino acids 377-1505: Prot-r-MAP2-P1, Prot-r-MAP2-P2 and Prot-r-MAP2-P3

storage :

Stable at 4 for at least one year

application details :

WB: 1:20,000. IF/ICC and IHC: 1:5,000-1:10,000.

applications :

IF, IHC, WB

reactivity :

Human, Mouse, Rat

image labels :

Western blot analysis of whole brain tissue lysates using chicken pAb to microtubule associated protein 2 (MAP2), CPCA-MAP2, dilution 1:50,000 in green: [1] protein standard (red), [2] adult rat brain, [3] embryonic E20 rat brain, [4] adult mouse brain. Strong band at ~280kDa mark corresponds to two major isoforms of MAP2 protein referred to as MAP2A and MAP2B. Smaller fragments of these isoforms are also detected if the antibody is used at higher concentrations. Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with chicken pAb to microtubule associated protein 2 (MAP2), cpca-map2, dilution 1:10,000 in red, and mouse mAb to MAP-tau, dilution 1:2,000, in green. The blue is DAPI staining of nuclear DNA. cpca-map2 antibody stains dendrites and perikarya of neurons, while M01201-3 antibody labels neuronal perikarya, dendrites and also axonal process. As a result perikarya and dendrites appears orange-yellow, since they contain both MAP2 and tau, while axons are green.

research category :

Neural Stem Cells, Neuron Marker, Neuroscience, Signal Transduction, Stem Cells

synonyms :

Microtubule-associated protein 2;MAP-2;MAP2;

gene full name :

Microtubule-associated protein 2

molecular weight :

199526 MW

protein function :

The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.

subcellular localization :

Cytoplasm, cytoskeleton . Cell projection, dendrite .

last modified :

2/6/19 22:57