Anti-HLADR Antibody (Monoclonal, L243 ) | Boster M01195 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-HLADR Antibody (Monoclonal, L243 )

catalog :

M01195

quantity :

100 ug

price :

250 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

L243

reactivity :

human

application :

flow cytometry

product information

sku :

M01195

name :

Anti-HLADR Antibody (Monoclonal, L243 )

price :

250 USD

immunogen :

Human lymphoblastoid cell line.

form :

Liquid

host :

Mouse

contents :

100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide.

clonality :

Monoclonal

description :

Mouse IgG2a Kappa Monoclonal antibody for HLA-DRA detection. Tested with Flow Cytometry in Human. Various direct flourescent conjugates are available for FCM upon request. Please contact us for details.

size :

100 ug

reactivity :

Human

applications :

Flow Cytometry

clone number :

L243

gene full name :

HLA class II histocompatibility antigen, DR alpha chain

gene name :

HLA-DRA

isotype :

IgG2a Kappa

protein function :

Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal microenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.

protein name :

HLA class II histocompatibility antigen, DR alpha chain

storage :

Store the antibody at 4 stable for 6 months. For long-term storage; store at -20 . Avoid repeated freeze and thaw cycles.

subcellular localization :

Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus, trans-Golgi network membrane; Single-pass type I membrane protein. Endosome membrane; Single- pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. Late endosome membrane; Single-pass type I membrane protein. The MHC class II complex transits through a number of intracellular compartments in the endocytic pathway until it reaches the cell membrane for antigen presentation.

synonyms :

HLA class II histocompatibility antigen, DR alpha chain;MHC class II antigen DRA;HLA-DRA;HLA-DRA1;

uniprot id :

P01903