Anti-Human CRM1 DyLight® 488 conjugated XPO1 Antibody (monoclonal, 5G3)

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-Human CRM1 DyLight® 488 conjugated XPO1 Antibody (monoclonal, 5G3)

catalog :

M01180-Dyl488

quantity :

50 ug/vial

clonality :

monoclonal

host :

mouse

conjugate :

DyLight488

clone name :

5G3

The same clone is also sold as:

Invitrogen: MA5-27879

reactivity :

human

application :

flow cytometry

product information

SKU :

M01180-Dyl488

Product Name :

Anti-Human CRM1 DyLight® 488 conjugated XPO1 Antibody (monoclonal, 5G3)

Size :

50 ug/vial

Clonality :

Monoclonal

Clone Number :

5G3

Host :

Mouse

Conjugate :

DyLight®488

Reactivity :

Human

Application(s) :

Flow Cytometry

Application Details :

Flow Cytometry, 1-3μg/1x10^6 cells

Description :

Boster Bio Anti-Human CRM1 DyLight® 488 conjugated XPO1 Antibody (monoclonal, 5G3) catalog # M01180-Dyl488. Tested in Flow Cytometry applications. This antibody reacts with Human.

Concentration :

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Gene Name :

XPO1

Uniprot ID :

O14980

Immunogen :

E.coli-derived human CRM1 recombinant protein (Position: N966-D1071). Human CRM1 shares 93.4% and 91.5% amino acid (aa) sequence identity with mouse and rat CRM1, respectively.

Form :

Liquid

Contents :

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.

Cross-reactivity :

No cross reactivity with other proteins.

Isotype :

IgG2b

Storage :

At 2-8?C for one year from date of receipt. Protect from light. Do not freeze.

Gene Full Name :

exportin 1

Synonyms :

Exportin-1; Exp1; Chromosome region maintenance 1 protein homolog; XPO1; CRM1

Protein Function :

Mediates the nuclear export of cellular proteins (cargos) bearing a leucine-rich nuclear export signal (NES) and of RNAs. In the nucleus, in association with RANBP3, binds cooperatively to the NES on its target protein and to the GTPase RAN in its active GTP-bound form (Ran-GTP). Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the cargo from the export receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Involved in U3 snoRNA transport from Cajal bodies to nucleoli. Binds to late precursor U3 snoRNA bearing a TMG cap. Several viruses, among them HIV-1, HTLV-1 and influenza A use it to export their unspliced or incompletely spliced RNAs out of the nucleus. Interacts with, and mediates the nuclear export of HIV-1 Rev and HTLV-1 Rex proteins. Involved in HTLV-1 Rex multimerization.

Subcellular Localization :

Cytoplasm. Nucleus, nucleoplasm. Nucleus, Cajal body. Nucleus, nucleolus. Located in the nucleoplasm, Cajal bodies and nucleoli. Shuttles between the nucleus/nucleolus and the cytoplasm.

Tissue Specificity :

Expressed in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. Not expressed in the kidney.

Background :

Exportin 1 (XPO1), also known as chromosomal maintenance 1 (CRM1), is an eukaryotic protein that mapped to human chromosome 2p16 by fluorescence in situ hybridization. This protein mediates leucine-rich nuclear export signal (NES)-dependent protein transport. It specifically inhibits the nuclear export of Rev and U snRNAs. Additionally, this protein is involved in the control of several cellular processes by controlling the localization of cyclin B, MPAK, and MAPKAP kinase 2. It also regulates NFAT and AP-1.

Research Category :

DNA / RNA, Epigenetics and Nuclear Signaling, Nuclear Import / Export, Nuclear Receptors, Nuclear Signaling Pathways, Protein Trafficking, RNA Processing, Signal Transduction