Anti-DAP Kinase 1 DAPK1 Rabbit Monoclonal Antibody | Boster M01161 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-DAP Kinase 1 DAPK1 Rabbit Monoclonal Antibody

catalog :

M01161

quantity :

100 µl

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

COA-4

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

product information

SKU :

M01161

Product Name :

Anti-DAP Kinase 1 DAPK1 Rabbit Monoclonal Antibody

Size :

100 µl

Clonality :

Monoclonal

Clone Number :

COA-4

Host :

Rabbit

Reactivity :

Human, Mouse, Rat

Application(s) :

IF, IHC, ICC, WB

Application Details :

WB 1:500-1:2000 .IHC 1:50-1:200 .ICC/IF 1:50-1:200

Description :

Boster Bio Anti-DAP Kinase 1 DAPK1 Rabbit Monoclonal Antibody catalog # M01161. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Concentration :

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Gene Name :

DAPK1

Uniprot ID :

P53355

Immunogen :

A synthesized peptide derived from human DAP Kinase 1

Form :

Liquid

Contents :

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Purification :

Affinity-chromatography

Storage :

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Gene Full Name :

Death-associated protein kinase 1

Synonyms :

Death-associated protein kinase 1; DAP kinase 1; 2.7.11.1; DAPK1; DAPK;

Molecular Weight :

160046 MW

Protein Function :

Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy. Regulates both type I apoptotic and type II autophagic cell deaths signal, depending on the cellular setting. The former is caspase-dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Phosphorylates PIN1 resulting in inhibition of its catalytic activity, nuclear localization, and cellular function. Phosphorylates TPM1, enhancing stress fiber formation in endothelial cells. Phosphorylates STX1A and significantly decreases its binding to STXBP1. Phosphorylates PRKD1 and regulates JNK signaling by binding and activating PRKD1 under oxidative stress. Phosphorylates BECN1, reducing its interaction with BCL2 and BCL2L1 and promoting the induction of autophagy. Phosphorylates TSC2, disrupting the TSC1-TSC2 complex and stimulating mTORC1 activity in a growth factor-dependent pathway. Phosphorylates RPS6, MYL9 and DAPK3. Acts as a signaling amplifier of NMDA receptors at extrasynaptic sites for mediating brain damage in stroke. Cerebral ischemia recruits DAPK1 into the NMDA receptor complex and it phosphorylates GRINB at Ser-1303 inducing injurious Ca (2+) influx through NMDA receptor channels, resulting in an irreversible neuronal death. Required together with DAPK3 for phosphorylation of RPL13A upon interferon-gamma activation which is causing RPL13A involvement in transcript- selective translation inhibition.

Subcellular Localization :

Isoform 1: Cytoplasm. Cytoplasm, cytoskeleton. Colocalizes with MAP1B in the microtubules and cortical actin fibers.

Tissue Specificity :

Isoform 2 is expressed in normal intestinal tissue as well as in colorectal carcinomas.

Research Category :

Apoptosis, Calcium Signaling, Calmodulin Pathway, Cancer, Cell Biology, Intracellular, Kinases, Oncoproteins/Suppressors, Protein Phosphorylation, Ser / Thr Kinases, Signal Transduction, Signaling Pathway, Tumor Suppressors