Anti-alpha smooth muscle Actin ACTA2 Rabbit Monoclonal Antibody

product summary

company name :

Boster

product type :

antibody

product name :

Anti-alpha smooth muscle Actin ACTA2 Rabbit Monoclonal Antibody

catalog :

M01072-1

quantity :

100 µl

price :

315 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

EO-1

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry

more info or order :

Boster product webpage

citations: 1

Reference
Hoa N, Ge L, Korach K, Levin E. Estrogen receptor beta maintains expression of KLF15 to prevent cardiac myocyte hypertrophy in female rodents. Mol Cell Endocrinol. 2018;470:240-250 pubmed publisher

image

image 1 :

Western blot analysis of alpha smooth muscle Actin expression in (1)A549 cell lysate; (2)C2C12 cell lysate using alpha-SMA antibody (M01072-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACTA2 monoclonal antibody (Catalog # M01072-1) overnight at 4Â°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACTA2

image 2 :

Immunohistochemical analysis of paraffin-embedded human colon, using alpha smooth muscle Actin Antibody(M01072-1). ACTA2 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ACTA2 Antibody (M01072-1)overnight at 4Â°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37Â°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

image 3 :

IF analysis of immunocytochemical section of A431 cell using anti- alpha smooth muscle Actin antibody (M01072-1) . Alpha smooth muscle Actin was detected in immunocytochemical section. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/mL rabbit anti- alpha smooth muscle Actin Antibody (M01072-1) overnight at 4Â°C. DyLightÂ®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37Â°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

product information

SKU :

M01072-1

Product Name :

Anti-alpha smooth muscle Actin ACTA2 Rabbit Monoclonal Antibody

Price :

315 USD

Size :

100 µl

Clonality :

Monoclonal

Clone Number :

EO-1

Host :

Rabbit

Reactivity :

Human, Mouse, Rat

Application(s) :

Flow Cytometry, IF, IHC, ICC, WB

Application Details :

WB 1:1000-1:10000 .IHC 1:50-1:200 .ICC/IF 1:50-1:200 .Flow Cytometry 1:20

Description :

Boster Bio Anti-alpha smooth muscle Actin ACTA2 Rabbit Monoclonal Antibody catalog # M01072-1. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Concentration :

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Gene Name :

ACTA2

Uniprot ID :

P62736

Immunogen :

A synthesized peptide derived from human alpha smooth muscle Actin

Form :

Liquid

Contents :

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Purification :

Affinity-chromatography

Storage :

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Gene Full Name :

Actin, aortic smooth muscle

Synonyms :

Actin, aortic smooth muscle; Alpha-actin-2; Cell growth-inhibiting gene 46 protein; ACTA2; ACTSA, ACTVS; GIG46;

Molecular Weight :

42009 MW

Protein Function :

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Subcellular Localization :

Cytoplasm, cytoskeleton.

Research Category :

Actin, Actin Etc, Cytoskeleton, Cytoskeleton / Ecm, Microfilaments, Signal Transduction

more info or order :

Boster product webpage