Anti-hGH Purified GH1 Monoclonal Antibody | Boster M00851-2 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-hGH Purified GH1 Monoclonal Antibody

catalog :

M00851-2

quantity :

0.1 mg (C100)

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

GH-45

The same clone is also sold as:

OriGene: SM3088P
Abnova: MAB0868
Exbio: 11-126-C025, 11-126-C100
Santa Cruz Biotechnology: sc-51602

reactivity :

human

application :

immunohistochemistry, immunocytochemistry

product information

SKU :

M00851-2

Product Name :

Anti-hGH Purified GH1 Monoclonal Antibody

Size :

0.1 mg (C100)

Clonality :

Monoclonal

Clone Number :

GH-45

Host :

Mouse

Reactivity :

Human

Application(s) :

IHC, ICC, ELISA

Application Notes :

Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-hGH Purified GH1 Monoclonal Antibody catalog # M00851-2. Tested in ELISA, IHC, ICC applications. This antibody reacts with Human.

Concentration :

0.5 mg/ml

Gene Name :

GH1

Uniprot ID :

P01241

Immunogen :

Human growth hormone

Form :

Liquid

Contents :

Phosphate buffered saline (PBS) solution with 15 mM sodium azide

Purification :

Purified by protein-A affinity chromatography

Isotype :

Mouse IgG1

Storage :

Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label.

Gene Full Name :

RNA polymerase II subunit A

Synonyms :

DNA-directed RNA polymerase II subunit RPB1; RNA polymerase II subunit B1; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase III largest subunit; RNA-directed RNA polymerase II subunit RPB1; POLR2A; POLR2

Protein Function :

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing. Regulation of gene expression levels depends on the balance between methylation and acetylation levels of tha CTD-lysines. Initiation or early elongation steps of transcription of growth-factors-induced immediate early genes are regulated by the acetylation status of the CTD. Methylation and dimethylation have a repressive effect on target genes expression. (Microbial infection) Acts as an RNA-dependent RNA polymerase when associated with small delta antigen of Hepatitis delta virus, acting both as a replicate and transcriptase for the viral RNA circular genome.

Subcellular Localization :

Nucleus. Cytoplasm.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.

Background :

DNA-directed RNA polymerase II subunit RPB1, also known as RPB1, is an enzyme that in humans is encoded by the POLR2A gene. It is mapped to 17p13.1. This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.