Anti-DHFR Antibody Picoband™ (monoclonal, 3C8) | Boster M00813-1 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-DHFR Antibody Picoband™ (monoclonal, 3C8)

catalog :

M00813-1

quantity :

100µg/vial

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

3C8

The same clone is also sold as:

Invitrogen: MA5-49170

reactivity :

human, rat

application :

western blot, immunohistochemistry - paraffin section

product information

SKU :

M00813-1

Product Name :

Anti-DHFR Antibody Picoband™ (monoclonal, 3C8)

Size :

100µg/vial

Clonality :

Monoclonal

Clone Number :

3C8

Host :

Mouse

Reactivity :

Human, Rat

Application(s) :

WB, IHC-P

Application Details :

Western blot, 0.1-0.5µg/ml. Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml.

Description :

Boster Bio Anti-DHFR Antibody Picoband™ (monoclonal, 3C8) catalog # M00813-1. Tested in IHC-P, WB applications. This antibody reacts with Human, Rat.

Concentration :

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Gene Name :

DHFR

Uniprot ID :

P00374

Immunogen :

E.coli-derived human DHFR recombinant protein (Position: V2-D187). Human DHFR?shares?90%?amino?acid? (aa)?sequence?identity?with both mouse?and?rat DHFR.

Form :

Lyophilized

Contents :

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Cross-reactivity :

No cross reactivity with other proteins.

Isotype :

IgG2b

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500µg/ml.

Gene Full Name :

dihydrofolate reductase

Synonyms :

Dihydrofolate reductase; DHFR

Protein Function :

Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFR2.

Subcellular Localization :

Mitochondrion. Cytoplasm

Tissue Specificity :

Widely expressed in fetal and adult tissues, including throughout the fetal and adult brains and whole blood. Expression is higher in the adult brain than in the fetal brain.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).

Research Category :

Amino Acid Metabolism, Amino Acids, Cancer, Cell Biology, Metabolic Signaling Pathways, Metabolism, Nucleotide Metabolism, Pathways And Processes, Signal Transduction