Anti-Collagen III/COL3A1 Antibody Picoband™ (monoclonal, 9H9)

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-Collagen III/COL3A1 Antibody Picoband™ (monoclonal, 9H9)

catalog :

M00788

quantity :

100µg/vial

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

9H9

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

product information

SKU :

M00788

Product Name :

Anti-Collagen III/COL3A1 Antibody Picoband™ (monoclonal, 9H9)

Size :

100µg/vial

Clonality :

Monoclonal

Clone Number :

9H9

Host :

Mouse

Reactivity :

Human, Mouse, Rat

Application(s) :

WB, IHC-P

Application Details :

Western blot, 0.1-0.5µg/ml. Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml.

Description :

Boster Bio Anti-Collagen III/COL3A1 Antibody Picoband™ (monoclonal, 9H9) catalog # M00788. Tested in IHC-P, WB applications. This antibody reacts with Human, Mouse, Rat.

Concentration :

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Gene Name :

COL3A1

Uniprot ID :

P02461

Immunogen :

E. coli-derived human Collagen III/COL3A1 recombinant protein (Position: D1222- E1455).

Form :

Lyophilized

Contents :

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Cross-reactivity :

No cross reactivity with other proteins.

Isotype :

IgG2b

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500µg/ml.

Gene Full Name :

collagen, type III, alpha 1

Synonyms :

Collagen alpha-1 (III) chain; COL3A1

Protein Function :

Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of ADGRG1 in the developing brain and binding to ADGRG1 inhibits neuronal migration and activates the RhoA pathway by coupling ADGRG1 to GNA13 and possibly GNA12.

Subcellular Localization :

Extracellular matrix

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).

Background :

COL3A1, also called EDS4A or Collagen alpha-1 (III), is a protein that in humans is encoded by the COL3A1 gene. It is mapped to 2q32.2. COL3A1 chain is a fibrillar-forming collagen comprising 3 alpha-1 (III) chains and is expressed in early embryos and throughout embryogenesis. In adult, COL3A1 is a major component of the extracellular matrix in a variety of internal organs and skin. COL3A1 is also a fibrous scleroprotein in bone, cartilage, dentin, tendon, bone marrow stroma and other connective tissue. It is involved in regulation of cortical development, and it is the major ligand of GPR56 in the developing brain. COL3A1 binding to GPR56 can inhibit neuronal migration and activate the RhoA pathway by coupling GPR56 to GNA13 and possibly GNA12.

Research Category :

Collagen, Cytoskeleton / Ecm, Ecm Proteins, Extracellular Matrix, Mesenchymal Stem Cells, Signal Transduction, Stem Cells