Anti-LAMP1 Monoclonal Antibody | Boster M00780-1 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-LAMP1 Monoclonal Antibody

catalog :

M00780-1

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

Ly1C6

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, immunoprecipitation

product information

sku :

M00780-1

status :

Enabled

name :

Anti-LAMP1 Monoclonal Antibody

category name :

Primary Antibodies, Monoclonal Antibodies, IHC ICC IF Antibodies

gene name :

Lamp1

price various sizes :

100ug / $299 100ug+Free HRP Secondary BA1050 / $299 100ug+Free Biotin Secondary BA1001 / $299

clonality :

Monoclonal

clone number :

Ly1C6

concentration :

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

conjugate :

contents :

Antibody concentration: 1 mg/ml, stored in PBS pH7.4, 50% glycerol, 0.09% sodium azide

description :

Mouse Monoclonal Ly1C6 antibody for Lamp1 detection. Tested positive for WB IF/IF IP in Human Mouse Rat Hamster

short description :

Mouse Monoclonal Ly1C6 antibody for Lamp1 detection. Tested positive for WB IF/IF IP in Human Mouse Rat Hamster

size :

100 ug

uniprot id :

P14562

host :

Mouse

immunogen :

Rat liver lysosomal membrane preparations

form :

liquid

purification :

Protein G Purified

application details :

WB (1:1000), ICC/IF (1:1000) ; optimal dilutions for assays should be determined by the user.

applications :

IP, IF, WB

reactivity :

Human, Mouse, Rat

image labels :

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-LAMPCali, San Fran School of Medicine. Figure 2. Western blot analysis of Lamp1 using anti-Lamp1 antibody (M00780-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lamp1 antigen affinity purified polyclonal antibody (Catalog # M00780-1) at 0.5 ug/mL overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1021) with Tanon 5200 system. A specific band was detected for Lamp1.

research category :

Cancer, Cell Death, Cell Type Marker, Hematopoietic Progenitors, Lymphoid, Metabolism, Metabolism Processes, Myeloid, Neurogenesis, Neurology Process, Neuron Marker, Neuroscience, Organelle Proteins, Organelles, Pathways And Processes, Protein Trafficking, Signal Transduction, Stem Cells, Subcellular Markers, T Lymphocytic Lineage, Tags & Cell Markers

last modified :

2/6/19 22:57