antibody

Anti-DDX3 Antibody (Monoclonal, ABM27H2)

M00751

100 ug

monoclonal

mouse

nonconjugated

ABM27H2

human, mouse

Enabled

Anti-DDX3 Antibody (Monoclonal, ABM27H2)

Primary Antibodies, Monoclonal Antibodies

DDX3X

25ug sample size / $99 100 ug / $299 100 ug+Free HRP Secondary BA1050 / $299 100 ug+Free Biotin Secondary BA1001 / $299

Monoclonal

ABM27H2

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

No

100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide.

Monoclonal clone# G2 antibody for DDX3/DDX3X detection. Host: Mouse.Size: 100 ug. Tested applications: WB. Reactive species: Human. DDX3/DDX3X information: Molecular Weight: 73243 MW; Subcellular Localization: Nucleus speckle. Cytoplasm. Mitochondrion outer membrane. Located predominantly in nuclear speckles and, at low levels, throughout the cytoplasm. Located to the outer side of nuclear pore complexes (NPC). Shuttles between the nucleus and the cytoplasm in a XPO1 and may be also in a NFX1-dependent manner. Associated with polyadenylated mRNAs in the cytoplasm and the nucleus. Predominantly located in nucleus during G(0) phase and in the cytoplasm during G1/S phase.

Mouse IgG2b Kappa Monoclonal antibody for DDX3X detection. Tested with WB in Human, Mouse.

100 ug

25ug for $99, contact us for details

O00571

Mouse

A partial length recombinant DDX3 protein (amino acids 10-230).

Liquid

Store the antibody at 4 C, stable for 6 months. For long-term storage, store at -20 C. Avoid repeated freeze and thaw cycles.

IgG2b Kappa

Western blot analysis, 2-4 ug/ml.

WB

Human, Mouse

Western blot analysis of DDX3. Anti- DDX3 antibody (Clone: ABM27H2) was used at 2 ug/ml on (1) MCF-7,(2) NIH3T3 (3) HEK293 and (4) EL-4 lysates.

sc 130736

Developmental Biology, Dna / Rna, Embryogenesis, Embryonic Stem Cells, Epigenetics And Nuclear Signaling, Intracellular, Rna Processing, Splicing, Stem Cells, Surface Molecules, Translation

ATP-dependent RNA helicase DDX3X;3.6.4.13;DEAD box protein 3, X-chromosomal;DEAD box, X isoform;Helicase-like protein 2;HLP2;DDX3X;DBX, DDX3;

ATP-dependent RNA helicase DDX3X

73243 MW

Multifunctional ATP-dependent RNA helicase. The ATPase activity can be stimulated by various ribo- and deoxynucleic acids indicative for a relaxed substrate specificity. In vitro can unwind partially double-stranded DNA with a preference for 5'- single-stranded DNA overhangs. Is involved in several steps of gene expression, such as transcription, mRNA maturation, mRNA export and translation. However, the exact mechanisms are not known and some functions may be specific for a subset of mRNAs. Involved in transcriptional regulation. Can enhance transcription from the CDKN1A/WAF1 promoter in a SP1-dependent manner. Found associated with the E-cadherin promoter and can down-regulate transcription from the promoter. Involved in regulation of translation initiation. Proposed to be involved in positive regulation of translation such as of cyclin E1/CCNE1 mRNA and specifically of mRNAs containing complex secondary structures in their 5'UTRs; these functions seem to require RNA helicase activity. Specifically promotes translation of a subset of viral and cellular mRNAs carrying a 5'proximal stem-loop structure in their 5'UTRs and cooperates with the eIF4F complex. Proposed to act prior to 43S ribosomal scanning and to locally destabilize these RNA structures to allow recognition of the mRNA cap or loading onto the 40S subunit. After association with 40S ribosomal subunits seems to be involved in the functional assembly of 80S ribosomes; the function seems to cover translation of mRNAs with structured and non-structured 5'UTRs and is independent of RNA helicase activity. Also proposed to inhibit cap-dependent translation by competetive interaction with EIF4E which can block the EIF4E:EIF4G complex formation. Proposed to be involved in stress response and stress granule assembly; the function is independent of RNA helicase activity and seems to involve association with EIF4E. May be involved in nuclear export of specific mRNAs but not in bulk mRNA export via interactions with XPO1 and NXF1. Also associates with polyadenylated mRNAs independently of NXF1. Associates with spliced mRNAs in an exon junction complex (EJC)-dependent manner and seems not to be directly involved in splicing. May be involved in nuclear mRNA export by association with DDX5 and regulating its nuclear location. Involved in innate immune signaling promoting the production of type I interferon (IFN-alpha and IFN-beta); proposed to act as viral RNA sensor, signaling intermediate and transcriptional coactivator. Involved in TBK1 and IKBKE-dependent IRF3 activation leading to IFNB induction, plays a role of scaffolding adapter that links IKBKE and IRF3 and coordinates their activation. Also found associated with IFNB promoters; the function is independent of IRF3. Can bind to viral RNAs and via association with MAVS/IPS1 and DDX58/RIG-I is thought to induce signaling in early stages of infection. Involved in regulation of apoptosis. May be required for activation of the intrinsic but inhibit activation of the extrinsic apoptotic pathway. Acts as an antiapoptotic protein through association with GSK3A/B and BIRC2 in an apoptosis antagonizing signaling complex; activation of death receptors promotes caspase-dependent cleavage of BIRC2 and DDX3X and relieves the inhibition. May be involved in mitotic chromosome segregation. Appears to be a prime target for viral manipulations. Hepatitis B virus (HBV) polymerase and possibly vaccinia virus (VACV) protein K7 inhibit IFNB induction probably by dissociating DDX3X from TBK1 or IKBKE. Is involved in hepatitis C virus (HCV) replication; the function may involve the association with HCV core protein. HCV core protein inhibits the IPS1-dependent function in viral RNA sensing and may switch the function from a INFB inducing to a HCV replication mode. Involved in HIV-1 replication. Acts as a cofactor for XPO1-mediated nuclear export of incompletely spliced HIV-1 Rev RNAs.

Nucleus speckle. Cytoplasm. Mitochondrion outer membrane. Located predominantly in nuclear speckles and, at low levels, throughout the cytoplasm. Located to the outer side of nuclear pore complexes (NPC). Shuttles between the nucleus and the cytoplasm in a XPO1 and may be also in a NFX1-dependent manner. Associated with polyadenylated mRNAs in the cytoplasm and the nucleus. Predominantly located in nucleus during G(0) phase and in the cytoplasm during G1/S phase.

ATP-dependent RNA helicase DDX3X

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