Anti-Emerin EMD Antibody Picoband™ (monoclonal, 5A10)

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-Emerin EMD Antibody Picoband™ (monoclonal, 5A10)

catalog :

M00714

quantity :

100µg/vial

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

5A10

The same clone is also sold as:

Invitrogen: MA5-27874

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry

product information

SKU :

M00714

Product Name :

Anti-Emerin EMD Antibody Picoband™ (monoclonal, 5A10)

Size :

100µg/vial

Clonality :

Monoclonal

Clone Number :

5A10

Host :

Mouse

Reactivity :

Human

Application(s) :

Flow Cytometry, IHC, ICC, WB

Application Details :

Western blot, 0.1-0.5µg/ml. Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml. Immunohistochemistry (Frozen Section), 0.5-1µg/ml. Immunocytochemistry, 0.5-1µg/ml. Flow Cytometry, 1-3μg/1x10^6 cells.

Description :

Boster Bio Anti-Emerin EMD Antibody Picoband™ (monoclonal, 5A10) catalog # M00714. Tested in Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human.

Concentration :

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Gene Name :

EMD

Uniprot ID :

P50402

Immunogen :

different from the related mouse sequence by eight amino acids, and from the related rat sequence by nine amino ac
MDNYADLSDTELTTLLRRYNIPHGPVVGSTRRLYEKKIF
EYETQRRRL),
A synthetic peptide corresponding to a sequence at the N-terminus of human Emerin (1-48aa

Form :

Lyophilized

Contents :

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Cross-reactivity :

No cross reactivity with other proteins.

Isotype :

IgG1

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Gene Full Name :

emerin

Synonyms :

Emerin; EMD; EDMD; STA

Protein Function :

Stabilizes and promotes the formation of a nuclear actin cortical network. Stimulates actin polymerization in vitro by binding and stabilizing the pointed end of growing filaments. Inhibits beta-catenin activity by preventing its accumulation in the nucleus. Acts by influencing the nuclear accumulation of beta- catenin through a CRM1-dependent export pathway. Links centrosomes to the nuclear envelope via a microtubule association. EMD and BAF are cooperative cofactors of HIV-1 infection. Association of EMD with the viral DNA requires the presence of BAF and viral integrase. The association of viral DNA with chromatin requires the presence of BAF and EMD. Required for proper localization of non-farnesylated prelamin-A/C.

Subcellular Localization :

Nucleus inner membrane

Tissue Specificity :

Skeletal muscle, heart, colon, testis, ovary and pancreas.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P), IHC(F) and ICC.

Research Category :

Biochemicals, Chemical Type, Neuroscience, Neurotransmission, Obesity, Receptors / Channels