Anti-GATA3 Rabbit Monoclonal Antibody | Boster M00593 product information

product summary

company name :

Boster

product type :

antibody

product name :

Anti-GATA3 Rabbit Monoclonal Antibody

catalog :

M00593

quantity :

100 µl

price :

315 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

ABBH-7

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry, chromatin immunoprecipitation

more info or order :

Boster product webpage

image

image 1 :

Western blot analysis of GATA3 expression in (1) SH-SY5Y cell lysate; (2) RAW 2647 cell lysate (M00593). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GATA3 monoclonal antibody (Catalog # M00593) overnight at 4Â°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GATA3

image 2 :

IHC analysis of GATA3 using anti-GATA3 antibody (M00593) on human bladder cancer. GATA3 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-GATA3 Antibody (M00593) overnight at 4Â°C. Biotinylated goat anti Rabbit IgG IgG antibody was used as secondary antibody and incubated for 30 minutes at 37Â°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

product information

SKU :

M00593

Product Name :

Anti-GATA3 Rabbit Monoclonal Antibody

Price :

315 USD

Size :

100 µl

Clonality :

Monoclonal

Clone Number :

ABBH-7

Host :

Rabbit

Reactivity :

Human

Application(s) :

ChIP, Flow Cytometry, IF, IHC, ICC, WB

Application Details :

WB 1:500-1:2000 .IHC 1:50-1:200 .ICC/IF 1:50-1:200 .Flow Cytometry 1:100 .

Description :

Boster Bio Anti-GATA3 Rabbit Monoclonal Antibody catalog # M00593. Tested in ChIP, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human.

Concentration :

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Gene Name :

GATA3

Uniprot ID :

P23771

Immunogen :

A synthesized peptide derived from human GATA3

Form :

Liquid

Contents :

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Purification :

Affinity-chromatography

Storage :

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Gene Full Name :

Trans-acting T-cell-specific transcription factor GATA-3

Synonyms :

Trans-acting T-cell-specific transcription factor GATA-3; GATA-binding factor 3; GATA3;

Molecular Weight :

47916 MW

Protein Function :

Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'. Required for the T-helper 2 (Th2) differentiation process following immune and inflammatory responses.

Subcellular Localization :

Nucleus.

Tissue Specificity :

T-cells and endothelial cells.

Research Category :

Adaptive Immunity, ChIP’ing Antibodies, Developmental Biology, Domain Families, Epigenetics and Nuclear Signaling, Immunology, Lineage Markers, Lineage Specification, Non-Cd, Stem Cells, T Cells, Transcription, Zinc Finger

more info or order :

Boster product webpage