Anti-MSH6/Gtbp Rabbit Monoclonal Antibody | Boster M00553 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-MSH6/Gtbp Rabbit Monoclonal Antibody

catalog :

M00553

quantity :

100 µl

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

AEB-13

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

product information

SKU :

M00553

Product Name :

Anti-MSH6/Gtbp Rabbit Monoclonal Antibody

Size :

100 µl

Clonality :

Monoclonal

Clone Number :

AEB-13

Host :

Rabbit

Reactivity :

Human, Mouse, Rat

Application(s) :

IF, IHC, ICC, WB

Application Details :

WB 1:500-1:2000 .IHC 1:50-1:200 .ICC/IF 1:50-1:200

Description :

Boster Bio Anti-MSH6/Gtbp Rabbit Monoclonal Antibody catalog # M00553. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Concentration :

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Gene Name :

MSH6

Uniprot ID :

P52701

Immunogen :

A synthesized peptide derived from human MSH6

Form :

Liquid

Contents :

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Purification :

Affinity-chromatography

Storage :

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Gene Full Name :

DNA mismatch repair protein Msh6

Synonyms :

DNA mismatch repair protein Msh6 ; hMSH6 ; G/T mismatch-binding protein ; GTBP ; GTMBP ; MutS protein homolog 6 ; MutS-alpha 160 kDa subunit; p160 ; MSH6 ; GTBP;

Molecular Weight :

152786 MW

Protein Function :

Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. Recruited on chromatin in G1 and early S phase via its PWWP domain that specifically binds trimethylated 'Lys-36' of histone H3 (H3K36me3): early recruitment to chromatin to be replicated allowing a quick identification of mismatch repair to initiate the DNA mismatch repair reaction.

Subcellular Localization :

Nucleus. Chromosome. Associates with H3K36me3 via its PWWP domain.

Research Category :

DNA / RNA, DNA Damage & Repair, Epigenetics and Nuclear Signaling